2etw: Difference between revisions

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[[Image:2etw.gif|left|200px]]<br /><applet load="2etw" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2etw.gif|left|200px]]<br /><applet load="2etw" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2etw, resolution 1.670Å" />

'''Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes'''<br />

==Overview==

The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds, a DNA consensus sequence (the middle sporulation element [MSE]) to, activate gene expression after the successful completion of meiotic, recombination. Here we report the X-ray crystal structures of Ndt80 bound, to ten distinct MSE variants. Comparison of these structures with the, structure of Ndt80 bound to a consensus MSE reveals structural principles, that determine the DNA binding specificity of this transcription factor., The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are, recognized by arginine side chains through a combination of hydrogen, bonding and cation-pi interactions. The 3' AT-rich region is recognized, via minor groove contacts that sterically exclude the N2 atom of GC base, pairs. The conformation of the AT-rich region is fixed by interactions, with the protein that favor recognition of poly(A)-poly(T) versus mixed AT, sequences through an avoidance of major groove steric clashes at 5'-ApT-3', steps.

The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds a DNA consensus sequence (the middle sporulation element [MSE]) to activate gene expression after the successful completion of meiotic recombination. Here we report the X-ray crystal structures of Ndt80 bound to ten distinct MSE variants. Comparison of these structures with the structure of Ndt80 bound to a consensus MSE reveals structural principles that determine the DNA binding specificity of this transcription factor. The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are recognized by arginine side chains through a combination of hydrogen bonding and cation-pi interactions. The 3' AT-rich region is recognized via minor groove contacts that sterically exclude the N2 atom of GC base pairs. The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps.

==About this Structure==

2ETW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2ETW OCA].

2ETW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ETW OCA].

==Reference==

Line 13:

[[Category: Saccharomyces cerevisiae]]

[[Category: Single protein]]

[[Category: Glover, J.N.]]

[[Category: Glover, J N.]]

[[Category: Lamoureux, J.S.]]

[[Category: Lamoureux, J S.]]

[[Category: b-dna]]

[[Category: beta sandwich]]

[[Category: ig-fold]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 10:08:~~19 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:14:33 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2etw.gif|left|200px]]<br /><applet load="2etw" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2etw.gif|left|200px]]<br /><applet load="2etw" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2etw, resolution 1.670&Aring;" />
 '''Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes'''<br />
 ==Overview==
-The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds, a DNA consensus sequence (the middle sporulation element [MSE]) to, activate gene expression after the successful completion of meiotic, recombination. Here we report the X-ray crystal structures of Ndt80 bound, to ten distinct MSE variants. Comparison of these structures with the, structure of Ndt80 bound to a consensus MSE reveals structural principles, that determine the DNA binding specificity of this transcription factor., The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are, recognized by arginine side chains through a combination of hydrogen, bonding and cation-pi interactions. The 3' AT-rich region is recognized, via minor groove contacts that sterically exclude the N2 atom of GC base, pairs. The conformation of the AT-rich region is fixed by interactions, with the protein that favor recognition of poly(A)-poly(T) versus mixed AT, sequences through an avoidance of major groove steric clashes at 5'-ApT-3', steps.
+The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds a DNA consensus sequence (the middle sporulation element [MSE]) to activate gene expression after the successful completion of meiotic recombination. Here we report the X-ray crystal structures of Ndt80 bound to ten distinct MSE variants. Comparison of these structures with the structure of Ndt80 bound to a consensus MSE reveals structural principles that determine the DNA binding specificity of this transcription factor. The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are recognized by arginine side chains through a combination of hydrogen bonding and cation-pi interactions. The 3' AT-rich region is recognized via minor groove contacts that sterically exclude the N2 atom of GC base pairs. The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps.
 ==About this Structure==
-ETW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ETW OCA].
+ETW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ETW OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
-[[Category: Glover, J.N.]]
+[[Category: Glover, J N.]]
-[[Category: Lamoureux, J.S.]]
+[[Category: Lamoureux, J S.]]
 [[Category: b-dna]]
 [[Category: beta sandwich]]
 [[Category: ig-fold]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:08:19 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:14:33 2008''