2f2h: Difference between revisions
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New page: left|200px<br /><applet load="2f2h" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f2h, resolution 1.950Å" /> '''Structure of the Yi... |
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[[Image:2f2h.gif|left|200px]]<br /><applet load="2f2h" size=" | [[Image:2f2h.gif|left|200px]]<br /><applet load="2f2h" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2f2h, resolution 1.950Å" /> | caption="2f2h, resolution 1.950Å" /> | ||
'''Structure of the YicI thiosugar Michaelis complex'''<br /> | '''Structure of the YicI thiosugar Michaelis complex'''<br /> | ||
==Overview== | ==Overview== | ||
For the first time, the thioglycoligase strategy has been successfully applied to -glycosidases. The -thioglycoligases derived from the family 31 glycosidases, -xylosidase from E. coli (YicI) and -glucosidase from Sulfolobus solfataricus, catalyze thioglycoligase reactions using -glycosyl fluorides and deoxythioglycosides as donors and acceptors, respectively, in yields up to 86%. In addition, we describe the Michaelis complex of YicI using one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss the structural insights this yields into the specificity and mechanism of family 31 -glycosidases and the molecular basis of an associated genetic disease. | For the first time, the thioglycoligase strategy has been successfully applied to alpha-glycosidases. The alpha-thioglycoligases derived from the family 31 glycosidases, alpha-xylosidase from E. coli (YicI) and alpha-glucosidase from Sulfolobus solfataricus, catalyze thioglycoligase reactions using alpha-glycosyl fluorides and deoxythioglycosides as donors and acceptors, respectively, in yields up to 86%. In addition, we describe the Michaelis complex of YicI using one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss the structural insights this yields into the specificity and mechanism of family 31 alpha-glycosidases and the molecular basis of an associated genetic disease. | ||
==About this Structure== | ==About this Structure== | ||
2F2H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, MPO, XTG and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2F2H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MPO:'>MPO</scene>, <scene name='pdbligand=XTG:'>XTG</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F2H OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Kim, Y | [[Category: Kim, Y W.]] | ||
[[Category: Lovering, A | [[Category: Lovering, A L.]] | ||
[[Category: Strynadka, N | [[Category: Strynadka, N C.J.]] | ||
[[Category: Withers, S | [[Category: Withers, S G.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: MPO]] | [[Category: MPO]] | ||
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[[Category: beta8alpha8 barrel]] | [[Category: beta8alpha8 barrel]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:17:01 2008'' | ||
