2f2q: Difference between revisions
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New page: left|200px<br /><applet load="2f2q" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f2q, resolution 1.45Å" /> '''High resolution crys... |
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[[Image:2f2q.gif|left|200px]]<br /><applet load="2f2q" size=" | [[Image:2f2q.gif|left|200px]]<br /><applet load="2f2q" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2f2q, resolution 1.45Å" /> | caption="2f2q, resolution 1.45Å" /> | ||
'''High resolution crystal strcuture of T4 lysosyme mutant L20R63/A liganded to guanidinium ion'''<br /> | '''High resolution crystal strcuture of T4 lysosyme mutant L20R63/A liganded to guanidinium ion'''<br /> | ||
==Overview== | ==Overview== | ||
The binding of guanidinium ion has been shown to promote a large-scale | The binding of guanidinium ion has been shown to promote a large-scale translation of a tandemly duplicated helix in an engineered mutant of T4 lysozyme. The guanidinium ion acts as a surrogate for the guanidino group of an arginine side chain. Here we determine whether methyl- and ethylguanidinium provide better mimics. The results show that addition of the hydrophobic moieties to the ligand enhances the binding affinity concomitant with reduction in ligand solubility. Crystallographic analysis confirms that binding of the alternative ligands to the engineered site still drives the large-scale conformational change. Thermal analysis and NMR data show, in comparison to guanidinium, an increase in protein stability and in ligand affinity. This is presumably due to the successive increase in hydrophobicity in going from guanidinium to ethylguanidinium. A fluorescence-based optical method was developed to sense the ligand-triggered helix translation in solution. The results are a first step in the de novo design of a molecular switch that is not related to the normal function of the protein. | ||
==About this Structure== | ==About this Structure== | ||
2F2Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with CL, GAI and HED as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | 2F2Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=GAI:'>GAI</scene> and <scene name='pdbligand=HED:'>HED</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F2Q OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Baase, W | [[Category: Baase, W A.]] | ||
[[Category: Bischoff, N.]] | [[Category: Bischoff, N.]] | ||
[[Category: Dyer, C | [[Category: Dyer, C M.]] | ||
[[Category: Matthews, B | [[Category: Matthews, B W.]] | ||
[[Category: Yousef, M | [[Category: Yousef, M S.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
[[Category: GAI]] | [[Category: GAI]] | ||
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[[Category: t4 lysozyme]] | [[Category: t4 lysozyme]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:17:06 2008'' | ||
