2fbd: Difference between revisions
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New page: left|200px<br /><applet load="2fbd" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fbd, resolution 1.90Å" /> '''The crystallographic... |
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[[Image:2fbd.jpg|left|200px]]<br /><applet load="2fbd" size=" | [[Image:2fbd.jpg|left|200px]]<br /><applet load="2fbd" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2fbd, resolution 1.90Å" /> | caption="2fbd, resolution 1.90Å" /> | ||
'''The crystallographic structure of the digestive lysozyme 1 from Musca domestica at 1.90 Ang.'''<br /> | '''The crystallographic structure of the digestive lysozyme 1 from Musca domestica at 1.90 Ang.'''<br /> | ||
==Overview== | ==Overview== | ||
Lysozymes are mostly known for their defensive role against bacteria, but | Lysozymes are mostly known for their defensive role against bacteria, but in several animals lysozymes have a digestive function. Here, the initial crystallographic characterization of two digestive lysozymes from Musca domestica are presented. The proteins were crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium sulfate or PEG/2-propanol as the precipitant. X-ray diffraction data were collected to a maximum resolution of 1.9 angstroms using synchrotron radiation. The lysozyme 1 and 2 crystals belong to the monoclinic space group P2(1) (unit-cell parameters a = 36.52, b = 79.44, c = 45.20 angstroms, beta = 102.97 degrees) and the orthorhombic space group P2(1)2(1)2 (unit-cell parameters a = 73.90, b = 96.40, c = 33.27 angstroms), respectively. The crystal structures were solved by molecular replacement and structure refinement is in progress. | ||
==About this Structure== | ==About this Structure== | ||
2FBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Musca_domestica Musca domestica] with SO4 and PEG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | 2FBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Musca_domestica Musca domestica] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=PEG:'>PEG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBD OCA]. | ||
==Reference== | ==Reference== | ||
Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica., Marana SR, Cancado FC, Valerio AA, Ferreira C, Terra WR, Barbosa JA, Acta | Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica., Marana SR, Cancado FC, Valerio AA, Ferreira C, Terra WR, Barbosa JA, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):750-2. Epub 2006 Jul 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16880547 16880547] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Musca domestica]] | [[Category: Musca domestica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Barbosa, J | [[Category: Barbosa, J A.R G.]] | ||
[[Category: Cancado, F | [[Category: Cancado, F C.]] | ||
[[Category: Marana, S | [[Category: Marana, S R.]] | ||
[[Category: PEG]] | [[Category: PEG]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: musca domestica]] | [[Category: musca domestica]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:19:39 2008'' | ||
