2fd4: Difference between revisions

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[[Image:2fd4.gif|left|200px]]<br /><applet load="2fd4" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2fd4.gif|left|200px]]<br /><applet load="2fd4" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2fd4, resolution 1.80Å" />

'''Crystal Structure of AvrPtoB (436-553)'''<br />

==Overview==

The Pseudomonas syringae protein AvrPtoB is translocated into plant cells, where it inhibits immunity-associated programmed cell death (PCD). The, structure of a C-terminal domain of AvrPtoB that is essential for anti-PCD, activity reveals an unexpected homology to the U-box and RING-finger, components of eukaryotic E3 ubiquitin ligases, and we show that AvrPtoB, has ubiquitin ligase activity. Mutation of conserved residues involved in, the binding of E2 ubiquitin-conjugating enzymes abolishes this activity in, vitro, as well as anti-PCD activity in tomato leaves, which dramatically, decreases virulence. These results show that Pseudomonas syringae uses a, mimic of host E3 ubiquitin ligases to inactivate plant defenses.

The Pseudomonas syringae protein AvrPtoB is translocated into plant cells, where it inhibits immunity-associated programmed cell death (PCD). The structure of a C-terminal domain of AvrPtoB that is essential for anti-PCD activity reveals an unexpected homology to the U-box and RING-finger components of eukaryotic E3 ubiquitin ligases, and we show that AvrPtoB has ubiquitin ligase activity. Mutation of conserved residues involved in the binding of E2 ubiquitin-conjugating enzymes abolishes this activity in vitro, as well as anti-PCD activity in tomato leaves, which dramatically decreases virulence. These results show that Pseudomonas syringae uses a mimic of host E3 ubiquitin ligases to inactivate plant defenses.

==About this Structure==

2FD4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2FD4 OCA].

2FD4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FD4 OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Janjusevic, R.]]

[[Category: Stebbins, C.E.]]

[[Category: Stebbins, C E.]]

[[Category: avrptob]]

[[Category: pseudomonas]]

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[[Category: ubiquitin ligase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 10:29:~~20 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:20:09 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2fd4.gif|left|200px]]<br /><applet load="2fd4" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2fd4.gif|left|200px]]<br /><applet load="2fd4" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2fd4, resolution 1.80&Aring;" />
 '''Crystal Structure of AvrPtoB (436-553)'''<br />
 ==Overview==
-The Pseudomonas syringae protein AvrPtoB is translocated into plant cells, where it inhibits immunity-associated programmed cell death (PCD). The, structure of a C-terminal domain of AvrPtoB that is essential for anti-PCD, activity reveals an unexpected homology to the U-box and RING-finger, components of eukaryotic E3 ubiquitin ligases, and we show that AvrPtoB, has ubiquitin ligase activity. Mutation of conserved residues involved in, the binding of E2 ubiquitin-conjugating enzymes abolishes this activity in, vitro, as well as anti-PCD activity in tomato leaves, which dramatically, decreases virulence. These results show that Pseudomonas syringae uses a, mimic of host E3 ubiquitin ligases to inactivate plant defenses.
+The Pseudomonas syringae protein AvrPtoB is translocated into plant cells, where it inhibits immunity-associated programmed cell death (PCD). The structure of a C-terminal domain of AvrPtoB that is essential for anti-PCD activity reveals an unexpected homology to the U-box and RING-finger components of eukaryotic E3 ubiquitin ligases, and we show that AvrPtoB has ubiquitin ligase activity. Mutation of conserved residues involved in the binding of E2 ubiquitin-conjugating enzymes abolishes this activity in vitro, as well as anti-PCD activity in tomato leaves, which dramatically decreases virulence. These results show that Pseudomonas syringae uses a mimic of host E3 ubiquitin ligases to inactivate plant defenses.
 ==About this Structure==
-FD4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FD4 OCA].
+FD4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FD4 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Janjusevic, R.]]
-[[Category: Stebbins, C.E.]]
+[[Category: Stebbins, C E.]]
 [[Category: avrptob]]
 [[Category: pseudomonas]]
@@ Line 21: / Line 21: @@
 [[Category: ubiquitin ligase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:29:20 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:20:09 2008''