2fok: Difference between revisions
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New page: left|200px<br /><applet load="2fok" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fok, resolution 2.30Å" /> '''STRUCTURE OF RESTRIC... |
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[[Image:2fok.jpg|left|200px]]<br /><applet load="2fok" size=" | [[Image:2fok.jpg|left|200px]]<br /><applet load="2fok" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2fok, resolution 2.30Å" /> | caption="2fok, resolution 2.30Å" /> | ||
'''STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI'''<br /> | '''STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI'''<br /> | ||
==Overview== | ==Overview== | ||
FokI is a member an unusual class of restriction enzymes that recognize a | FokI is a member an unusual class of restriction enzymes that recognize a specific DNA sequence and cleave nonspecifically a short distance away from that sequence. FokI consists of an N-terminal DNA recognition domain and a C-terminal cleavage domain. The bipartite nature of FokI has led to the development of artificial enzymes with novel specificities. We have solved the structure of FokI to 2.3 A resolution. The structure reveals a dimer, in which the dimerization interface is mediated by the cleavage domain. Each monomer has an overall conformation similar to that found in the FokI-DNA complex, with the cleavage domain packing alongside the DNA recognition domain. In corroboration with the cleavage data presented in the accompanying paper in this issue of Proceedings, we propose a model for FokI DNA cleavage that requires the dimerization of FokI on DNA to cleave both DNA strands. | ||
==About this Structure== | ==About this Structure== | ||
2FOK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Planomicrobium_okeanokoites Planomicrobium okeanokoites]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http:// | 2FOK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Planomicrobium_okeanokoites Planomicrobium okeanokoites]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FOK OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Type II site-specific deoxyribonuclease]] | [[Category: Type II site-specific deoxyribonuclease]] | ||
[[Category: Aggarwal, A | [[Category: Aggarwal, A K.]] | ||
[[Category: Bitinaite, J.]] | [[Category: Bitinaite, J.]] | ||
[[Category: Schildkraut, I.]] | [[Category: Schildkraut, I.]] | ||
[[Category: Wah, D | [[Category: Wah, D A.]] | ||
[[Category: deoxyribonuclease]] | [[Category: deoxyribonuclease]] | ||
[[Category: dna cleavage]] | [[Category: dna cleavage]] | ||
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[[Category: type iis restriction endonuclease]] | [[Category: type iis restriction endonuclease]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:23:32 2008'' | ||
