2fv4: Difference between revisions

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[[Image:2fv4.gif|left|200px]]<br /><applet load="2fv4" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2fv4.gif|left|200px]]<br /><applet load="2fv4" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2fv4" />

'''NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain'''<br />

==Overview==

The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during, mitosis. It is an approximately 570 A long, rod-shaped assembly of four, proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions, at either end of a central shaft. The complex bridges from the, centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular, end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2, globular end. We report the atomic structures of the Spc24/Spc25 globular, domain, determined both by X-ray crystallography at 1.9 A resolution and, by NMR. Spc24 and Spc25 fold tightly together into a single globular, entity with pseudo-2-fold symmetry. Conserved residues line a common, hydrophobic core and the bottom of a cleft, indicating that the functional, orthologs from other eukaryotes will have the same structure and, suggesting a docking site for components of the inner kinetochore.

The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during mitosis. It is an approximately 570 A long, rod-shaped assembly of four proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions at either end of a central shaft. The complex bridges from the centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2 globular end. We report the atomic structures of the Spc24/Spc25 globular domain, determined both by X-ray crystallography at 1.9 A resolution and by NMR. Spc24 and Spc25 fold tightly together into a single globular entity with pseudo-2-fold symmetry. Conserved residues line a common hydrophobic core and the bottom of a cleft, indicating that the functional orthologs from other eukaryotes will have the same structure and suggesting a docking site for components of the inner kinetochore.

==About this Structure==

2FV4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2FV4 OCA].

2FV4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FV4 OCA].

==Reference==

Line 13:

[[Category: Protein complex]]

[[Category: Saccharomyces cerevisiae]]

[[Category: Chou, J.J.]]

[[Category: Chou, J J.]]

[[Category: Schnell, J.R.]]

[[Category: Schnell, J R.]]

[[Category: alpha-beta]]

[[Category: coiled-coil]]

[[Category: complex]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:23 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2fv4.gif|left|200px]]<br /><applet load="2fv4" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2fv4.gif|left|200px]]<br /><applet load="2fv4" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2fv4" />
 '''NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain'''<br />
 ==Overview==
-The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during, mitosis. It is an approximately 570 A long, rod-shaped assembly of four, proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions, at either end of a central shaft. The complex bridges from the, centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular, end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2, globular end. We report the atomic structures of the Spc24/Spc25 globular, domain, determined both by X-ray crystallography at 1.9 A resolution and, by NMR. Spc24 and Spc25 fold tightly together into a single globular, entity with pseudo-2-fold symmetry. Conserved residues line a common, hydrophobic core and the bottom of a cleft, indicating that the functional, orthologs from other eukaryotes will have the same structure and, suggesting a docking site for components of the inner kinetochore.
+The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during mitosis. It is an approximately 570 A long, rod-shaped assembly of four proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions at either end of a central shaft. The complex bridges from the centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2 globular end. We report the atomic structures of the Spc24/Spc25 globular domain, determined both by X-ray crystallography at 1.9 A resolution and by NMR. Spc24 and Spc25 fold tightly together into a single globular entity with pseudo-2-fold symmetry. Conserved residues line a common hydrophobic core and the bottom of a cleft, indicating that the functional orthologs from other eukaryotes will have the same structure and suggesting a docking site for components of the inner kinetochore.
 ==About this Structure==
-FV4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FV4 OCA].
+FV4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FV4 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Chou, J.J.]]
+[[Category: Chou, J J.]]
-[[Category: Schnell, J.R.]]
+[[Category: Schnell, J R.]]
 [[Category: alpha-beta]]
 [[Category: coiled-coil]]
 [[Category: complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:47:16 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:23 2008''