2gas: Difference between revisions

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[[Image:2gas.gif|left|200px]]<br /><applet load="2gas" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2gas.gif|left|200px]]<br /><applet load="2gas" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2gas, resolution 1.6Å" />

'''Crystal Structure of Isoflavone Reductase'''<br />

==Overview==

Isoflavonoids play important roles in plant defense and exhibit a range of, mammalian health-promoting activities. Isoflavone reductase (IFR), specifically recognizes isoflavones and catalyzes a stereospecific, NADPH-dependent reduction to (3R)-isoflavanone. The crystal structure of, Medicago sativa IFR with deletion of residues 39-47 has been determined at, 1.6A resolution. Structural analysis, molecular modeling and docking, and, comparison with the structures of other NADPH-dependent enzymes, defined, the putative binding sites for co-factor and substrate and potential key, residues for enzyme activity and substrate specificity. Further, mutagenesis has confirmed the role of Lys144 as a catalytic residue. This, study provides a structural basis for understanding the enzymatic, mechanism and substrate specificity of IFRs as well as the functions of, IFR-like proteins.

Isoflavonoids play important roles in plant defense and exhibit a range of mammalian health-promoting activities. Isoflavone reductase (IFR) specifically recognizes isoflavones and catalyzes a stereospecific NADPH-dependent reduction to (3R)-isoflavanone. The crystal structure of Medicago sativa IFR with deletion of residues 39-47 has been determined at 1.6A resolution. Structural analysis, molecular modeling and docking, and comparison with the structures of other NADPH-dependent enzymes, defined the putative binding sites for co-factor and substrate and potential key residues for enzyme activity and substrate specificity. Further mutagenesis has confirmed the role of Lys144 as a catalytic residue. This study provides a structural basis for understanding the enzymatic mechanism and substrate specificity of IFRs as well as the functions of IFR-like proteins.

==About this Structure==

2GAS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2GAS OCA].

2GAS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAS OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Chang, Z.]]

[[Category: Dixon, R.A.]]

[[Category: Dixon, R A.]]

[[Category: He, X.]]

[[Category: Lin, J.]]

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[[Category: nadph-dependent reductase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 11:05:~~04 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:29:51 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2gas.gif|left|200px]]<br /><applet load="2gas" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2gas.gif|left|200px]]<br /><applet load="2gas" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2gas, resolution 1.6&Aring;" />
 '''Crystal Structure of Isoflavone Reductase'''<br />
 ==Overview==
-Isoflavonoids play important roles in plant defense and exhibit a range of, mammalian health-promoting activities. Isoflavone reductase (IFR), specifically recognizes isoflavones and catalyzes a stereospecific, NADPH-dependent reduction to (3R)-isoflavanone. The crystal structure of, Medicago sativa IFR with deletion of residues 39-47 has been determined at, 1.6A resolution. Structural analysis, molecular modeling and docking, and, comparison with the structures of other NADPH-dependent enzymes, defined, the putative binding sites for co-factor and substrate and potential key, residues for enzyme activity and substrate specificity. Further, mutagenesis has confirmed the role of Lys144 as a catalytic residue. This, study provides a structural basis for understanding the enzymatic, mechanism and substrate specificity of IFRs as well as the functions of, IFR-like proteins.
+Isoflavonoids play important roles in plant defense and exhibit a range of mammalian health-promoting activities. Isoflavone reductase (IFR) specifically recognizes isoflavones and catalyzes a stereospecific NADPH-dependent reduction to (3R)-isoflavanone. The crystal structure of Medicago sativa IFR with deletion of residues 39-47 has been determined at 1.6A resolution. Structural analysis, molecular modeling and docking, and comparison with the structures of other NADPH-dependent enzymes, defined the putative binding sites for co-factor and substrate and potential key residues for enzyme activity and substrate specificity. Further mutagenesis has confirmed the role of Lys144 as a catalytic residue. This study provides a structural basis for understanding the enzymatic mechanism and substrate specificity of IFRs as well as the functions of IFR-like proteins.
 ==About this Structure==
-GAS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GAS OCA].
+GAS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAS OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Chang, Z.]]
-[[Category: Dixon, R.A.]]
+[[Category: Dixon, R A.]]
 [[Category: He, X.]]
 [[Category: Lin, J.]]
@@ Line 21: / Line 21: @@
 [[Category: nadph-dependent reductase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:05:04 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:29:51 2008''