2gjc: Difference between revisions

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[[Image:2gjc.gif|left|200px]]<br /><applet load="2gjc" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2gjc.gif|left|200px]]<br /><applet load="2gjc" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2gjc, resolution 1.82Å" />

'''Crystal Structure of Thiazole Synthase in Saccharomyces cerevisiae at 1.82 A resolution'''<br />

==Overview==

The structure of thiazole synthase (Thi4) from Saccharomyces cerevisiae, was determined to 1.8 A resolution. Thi4 exists as an octamer with two, monomers in the asymmetric unit. The structure reveals the presence of a, tightly bound adenosine, diphospho-5-(beta-ethyl)-4-methylthiazole-2-carboxylic acid at the active, site. The isolation of this reaction product identifies NAD as the most, likely precursor and provides the first mechanistic insights into the, biosynthesis of the thiamin thiazole in eukaryotes. Additionally, the Thi4, structure reveals the first protein structure with a GR(2) domain that, binds NAD instead of FAD, raising interesting questions about how this, protein evolved from a flavoenzyme to a NAD binding enzyme.

The structure of thiazole synthase (Thi4) from Saccharomyces cerevisiae was determined to 1.8 A resolution. Thi4 exists as an octamer with two monomers in the asymmetric unit. The structure reveals the presence of a tightly bound adenosine diphospho-5-(beta-ethyl)-4-methylthiazole-2-carboxylic acid at the active site. The isolation of this reaction product identifies NAD as the most likely precursor and provides the first mechanistic insights into the biosynthesis of the thiamin thiazole in eukaryotes. Additionally, the Thi4 structure reveals the first protein structure with a GR(2) domain that binds NAD instead of FAD, raising interesting questions about how this protein evolved from a flavoenzyme to a NAD binding enzyme.

==About this Structure==

2GJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with AHZ as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2GJC OCA].

2GJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=AHZ:'>AHZ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJC OCA].

==Reference==

Line 13:

[[Category: Saccharomyces cerevisiae]]

[[Category: Single protein]]

[[Category: Begley, T.P.]]

[[Category: Begley, T P.]]

[[Category: Chatterjee, A.]]

[[Category: Ealick, S.E.]]

[[Category: Ealick, S E.]]

[[Category: Jurgenson, C.T.]]

[[Category: Jurgenson, C T.]]

[[Category: AHZ]]

[[Category: glutathione reductase type ii family]]

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[[Category: thiazole synthase]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:32:14 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2gjc.gif|left|200px]]<br /><applet load="2gjc" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2gjc.gif|left|200px]]<br /><applet load="2gjc" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2gjc, resolution 1.82&Aring;" />
 '''Crystal Structure of Thiazole Synthase in Saccharomyces cerevisiae at 1.82 A resolution'''<br />
 ==Overview==
-The structure of thiazole synthase (Thi4) from Saccharomyces cerevisiae, was determined to 1.8 A resolution. Thi4 exists as an octamer with two, monomers in the asymmetric unit. The structure reveals the presence of a, tightly bound adenosine, diphospho-5-(beta-ethyl)-4-methylthiazole-2-carboxylic acid at the active, site. The isolation of this reaction product identifies NAD as the most, likely precursor and provides the first mechanistic insights into the, biosynthesis of the thiamin thiazole in eukaryotes. Additionally, the Thi4, structure reveals the first protein structure with a GR(2) domain that, binds NAD instead of FAD, raising interesting questions about how this, protein evolved from a flavoenzyme to a NAD binding enzyme.
+The structure of thiazole synthase (Thi4) from Saccharomyces cerevisiae was determined to 1.8 A resolution. Thi4 exists as an octamer with two monomers in the asymmetric unit. The structure reveals the presence of a tightly bound adenosine diphospho-5-(beta-ethyl)-4-methylthiazole-2-carboxylic acid at the active site. The isolation of this reaction product identifies NAD as the most likely precursor and provides the first mechanistic insights into the biosynthesis of the thiamin thiazole in eukaryotes. Additionally, the Thi4 structure reveals the first protein structure with a GR(2) domain that binds NAD instead of FAD, raising interesting questions about how this protein evolved from a flavoenzyme to a NAD binding enzyme.
 ==About this Structure==
-GJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with AHZ as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GJC OCA].
+GJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=AHZ:'>AHZ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJC OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
-[[Category: Begley, T.P.]]
+[[Category: Begley, T P.]]
 [[Category: Chatterjee, A.]]
-[[Category: Ealick, S.E.]]
+[[Category: Ealick, S E.]]
-[[Category: Jurgenson, C.T.]]
+[[Category: Jurgenson, C T.]]
 [[Category: AHZ]]
 [[Category: glutathione reductase type ii family]]
@@ Line 22: / Line 22: @@
 [[Category: thiazole synthase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:14:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:32:14 2008''