2gm4: Difference between revisions

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[[Image:2gm4.gif|left|200px]]<br /><applet load="2gm4" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2gm4.gif|left|200px]]<br /><applet load="2gm4" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2gm4, resolution 3.50Å" />

'''An activated, tetrameric gamma-delta resolvase: Hin chimaera bound to cleaved DNA'''<br />

==Overview==

The structures of two mutants of the site-specific recombinase, gammadelta, resolvase, that form activated tetramers have been determined. One, at, 3.5-A resolution, forms a synaptic intermediate of resolvase that is, covalently linked to two cleaved DNAs, whereas the other is of an, unliganded structure determined at 2.1-A resolution. Comparisons of the, four known tetrameric resolvase structures show that the subunits interact, through the formation of a common core of four helices. The N-terminal, halves of these helices superimpose well on each other, whereas the, orientations of their C termini are more variable. The catalytic domains, of resolvase in the unliganded structure are arranged asymmetrically, demonstrating that their positions can move substantially while preserving, the four-helix core that forms the tetramer. These results suggest that, the precleavage synaptic tetramer of gammadelta resolvase, whose structure, is not known, may be formed by a similar four-helix core, but differ in, the relative orientations of its catalytic and DNA-binding domains.

The structures of two mutants of the site-specific recombinase, gammadelta resolvase, that form activated tetramers have been determined. One, at 3.5-A resolution, forms a synaptic intermediate of resolvase that is covalently linked to two cleaved DNAs, whereas the other is of an unliganded structure determined at 2.1-A resolution. Comparisons of the four known tetrameric resolvase structures show that the subunits interact through the formation of a common core of four helices. The N-terminal halves of these helices superimpose well on each other, whereas the orientations of their C termini are more variable. The catalytic domains of resolvase in the unliganded structure are arranged asymmetrically, demonstrating that their positions can move substantially while preserving the four-helix core that forms the tetramer. These results suggest that the precleavage synaptic tetramer of gammadelta resolvase, whose structure is not known, may be formed by a similar four-helix core, but differ in the relative orientations of its catalytic and DNA-binding domains.

==About this Structure==

2GM4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2GM4 OCA].

2GM4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GM4 OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Ho, R.S.]]

[[Category: Ho, R S.]]

[[Category: Kamtekar, S.]]

[[Category: Li, W.]]

[[Category: Steitz, T.A.]]

[[Category: Steitz, T A.]]

[[Category: gamma delta resolvase]]

[[Category: protein dna complex]]

[[Category: site specific recombination]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 11:16:~~28 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:33:02 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2gm4.gif|left|200px]]<br /><applet load="2gm4" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2gm4.gif|left|200px]]<br /><applet load="2gm4" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2gm4, resolution 3.50&Aring;" />
 '''An activated, tetrameric gamma-delta resolvase: Hin chimaera bound to cleaved DNA'''<br />
 ==Overview==
-The structures of two mutants of the site-specific recombinase, gammadelta, resolvase, that form activated tetramers have been determined. One, at, 3.5-A resolution, forms a synaptic intermediate of resolvase that is, covalently linked to two cleaved DNAs, whereas the other is of an, unliganded structure determined at 2.1-A resolution. Comparisons of the, four known tetrameric resolvase structures show that the subunits interact, through the formation of a common core of four helices. The N-terminal, halves of these helices superimpose well on each other, whereas the, orientations of their C termini are more variable. The catalytic domains, of resolvase in the unliganded structure are arranged asymmetrically, demonstrating that their positions can move substantially while preserving, the four-helix core that forms the tetramer. These results suggest that, the precleavage synaptic tetramer of gammadelta resolvase, whose structure, is not known, may be formed by a similar four-helix core, but differ in, the relative orientations of its catalytic and DNA-binding domains.
+The structures of two mutants of the site-specific recombinase, gammadelta resolvase, that form activated tetramers have been determined. One, at 3.5-A resolution, forms a synaptic intermediate of resolvase that is covalently linked to two cleaved DNAs, whereas the other is of an unliganded structure determined at 2.1-A resolution. Comparisons of the four known tetrameric resolvase structures show that the subunits interact through the formation of a common core of four helices. The N-terminal halves of these helices superimpose well on each other, whereas the orientations of their C termini are more variable. The catalytic domains of resolvase in the unliganded structure are arranged asymmetrically, demonstrating that their positions can move substantially while preserving the four-helix core that forms the tetramer. These results suggest that the precleavage synaptic tetramer of gammadelta resolvase, whose structure is not known, may be formed by a similar four-helix core, but differ in the relative orientations of its catalytic and DNA-binding domains.
 ==About this Structure==
-GM4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GM4 OCA].
+GM4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GM4 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Ho, R.S.]]
+[[Category: Ho, R S.]]
 [[Category: Kamtekar, S.]]
 [[Category: Li, W.]]
-[[Category: Steitz, T.A.]]
+[[Category: Steitz, T A.]]
 [[Category: gamma delta resolvase]]
 [[Category: protein dna complex]]
 [[Category: site specific recombination]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:16:28 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:33:02 2008''