2go9: Difference between revisions

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[[Image:2go9.jpg|left|200px]]<br /><applet load="2go9" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2go9.jpg|left|200px]]<br /><applet load="2go9" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2go9" />

'''RRM domains 1 and 2 of Prp24 from S. cerevisiae'''<br />

==Overview==

The essential Saccharomyces cerevisiae pre-messenger RNA splicing protein, 24 (Prp24) has four RNA recognition motifs (RRMs) and facilitates U6 RNA, base-pairing with U4 RNA during spliceosome assembly. Prp24 is a component, of the free U6 small nuclear ribonucleoprotein particle (snRNP) but not, the U4/U6 bi-snRNP, and so is thought to be displaced from U6 by U4/U6, base-pairing. The interaction partners of each of the four RRMs of Prp24, and how these interactions direct U4/U6 pairing are not known. Here we, report the crystal structure of the first three RRMs and the solution, structure of the first two RRMs of Prp24. Strikingly, RRM 2 forms, extensive inter-domain contacts with RRMs 1 and 3. These contacts occupy, much of the canonical RNA-binding faces (beta-sheets) of RRMs 1 and 2, but, leave the beta-sheet of RRM 3 exposed. Previously identified substitutions, in Prp24 that suppress mutations in U4 and U6 spliceosomal RNAs cluster, primarily in the beta-sheet of RRM 3, but also in a conserved loop of RRM, 2. RNA binding assays and chemical shift mapping indicate that a large, basic patch evident on the surface of RRMs 1 and 2 is part of a high, affinity U6 RNA binding site. Our results suggest that Prp24 binds free U6, RNA primarily with RRMs 1 and 2, which may remodel the U6 secondary, structure. The beta-sheet of RRM 3 then influences U4/U6 pairing through, interaction with an unidentified ligand.

The essential Saccharomyces cerevisiae pre-messenger RNA splicing protein 24 (Prp24) has four RNA recognition motifs (RRMs) and facilitates U6 RNA base-pairing with U4 RNA during spliceosome assembly. Prp24 is a component of the free U6 small nuclear ribonucleoprotein particle (snRNP) but not the U4/U6 bi-snRNP, and so is thought to be displaced from U6 by U4/U6 base-pairing. The interaction partners of each of the four RRMs of Prp24 and how these interactions direct U4/U6 pairing are not known. Here we report the crystal structure of the first three RRMs and the solution structure of the first two RRMs of Prp24. Strikingly, RRM 2 forms extensive inter-domain contacts with RRMs 1 and 3. These contacts occupy much of the canonical RNA-binding faces (beta-sheets) of RRMs 1 and 2, but leave the beta-sheet of RRM 3 exposed. Previously identified substitutions in Prp24 that suppress mutations in U4 and U6 spliceosomal RNAs cluster primarily in the beta-sheet of RRM 3, but also in a conserved loop of RRM 2. RNA binding assays and chemical shift mapping indicate that a large basic patch evident on the surface of RRMs 1 and 2 is part of a high affinity U6 RNA binding site. Our results suggest that Prp24 binds free U6 RNA primarily with RRMs 1 and 2, which may remodel the U6 secondary structure. The beta-sheet of RRM 3 then influences U4/U6 pairing through interaction with an unidentified ligand.

==About this Structure==

2GO9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2GO9 OCA].

2GO9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GO9 OCA].

==Reference==

Line 13:

[[Category: Saccharomyces cerevisiae]]

[[Category: Single protein]]

[[Category: Brow, D.A.]]

[[Category: Brow, D A.]]

[[Category: Butcher, S.E.]]

[[Category: Butcher, S E.]]

[[Category: Kwan, S.K.]]

[[Category: Kwan, S K.]]

[[Category: Lee, D.H.]]

[[Category: Lee, D H.]]

[[Category: Reiter, N.J.]]

[[Category: Reiter, N J.]]

[[Category: Tonelli, M.]]

[[Category: beta-alpha-beta]]

Line 24:

[[Category: rrm]]

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@@ Line 1: / Line 1: @@
-[[Image:2go9.jpg|left|200px]]<br /><applet load="2go9" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2go9.jpg|left|200px]]<br /><applet load="2go9" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2go9" />
 '''RRM domains 1 and 2 of Prp24 from S. cerevisiae'''<br />
 ==Overview==
-The essential Saccharomyces cerevisiae pre-messenger RNA splicing protein, 24 (Prp24) has four RNA recognition motifs (RRMs) and facilitates U6 RNA, base-pairing with U4 RNA during spliceosome assembly. Prp24 is a component, of the free U6 small nuclear ribonucleoprotein particle (snRNP) but not, the U4/U6 bi-snRNP, and so is thought to be displaced from U6 by U4/U6, base-pairing. The interaction partners of each of the four RRMs of Prp24, and how these interactions direct U4/U6 pairing are not known. Here we, report the crystal structure of the first three RRMs and the solution, structure of the first two RRMs of Prp24. Strikingly, RRM 2 forms, extensive inter-domain contacts with RRMs 1 and 3. These contacts occupy, much of the canonical RNA-binding faces (beta-sheets) of RRMs 1 and 2, but, leave the beta-sheet of RRM 3 exposed. Previously identified substitutions, in Prp24 that suppress mutations in U4 and U6 spliceosomal RNAs cluster, primarily in the beta-sheet of RRM 3, but also in a conserved loop of RRM, 2. RNA binding assays and chemical shift mapping indicate that a large, basic patch evident on the surface of RRMs 1 and 2 is part of a high, affinity U6 RNA binding site. Our results suggest that Prp24 binds free U6, RNA primarily with RRMs 1 and 2, which may remodel the U6 secondary, structure. The beta-sheet of RRM 3 then influences U4/U6 pairing through, interaction with an unidentified ligand.
+The essential Saccharomyces cerevisiae pre-messenger RNA splicing protein 24 (Prp24) has four RNA recognition motifs (RRMs) and facilitates U6 RNA base-pairing with U4 RNA during spliceosome assembly. Prp24 is a component of the free U6 small nuclear ribonucleoprotein particle (snRNP) but not the U4/U6 bi-snRNP, and so is thought to be displaced from U6 by U4/U6 base-pairing. The interaction partners of each of the four RRMs of Prp24 and how these interactions direct U4/U6 pairing are not known. Here we report the crystal structure of the first three RRMs and the solution structure of the first two RRMs of Prp24. Strikingly, RRM 2 forms extensive inter-domain contacts with RRMs 1 and 3. These contacts occupy much of the canonical RNA-binding faces (beta-sheets) of RRMs 1 and 2, but leave the beta-sheet of RRM 3 exposed. Previously identified substitutions in Prp24 that suppress mutations in U4 and U6 spliceosomal RNAs cluster primarily in the beta-sheet of RRM 3, but also in a conserved loop of RRM 2. RNA binding assays and chemical shift mapping indicate that a large basic patch evident on the surface of RRMs 1 and 2 is part of a high affinity U6 RNA binding site. Our results suggest that Prp24 binds free U6 RNA primarily with RRMs 1 and 2, which may remodel the U6 secondary structure. The beta-sheet of RRM 3 then influences U4/U6 pairing through interaction with an unidentified ligand.
 ==About this Structure==
-GO9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GO9 OCA].
+GO9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GO9 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
-[[Category: Brow, D.A.]]
+[[Category: Brow, D A.]]
-[[Category: Butcher, S.E.]]
+[[Category: Butcher, S E.]]
-[[Category: Kwan, S.K.]]
+[[Category: Kwan, S K.]]
-[[Category: Lee, D.H.]]
+[[Category: Lee, D H.]]
-[[Category: Reiter, N.J.]]
+[[Category: Reiter, N J.]]
 [[Category: Tonelli, M.]]
 [[Category: beta-alpha-beta]]
@@ Line 24: / Line 24: @@
 [[Category: rrm]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:18:31 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:33:40 2008''