2gwl: Difference between revisions

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[[Image:2gwl.gif|left|200px]]<br /><applet load="2gwl" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2gwl.gif|left|200px]]<br /><applet load="2gwl" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2gwl, resolution 1.900Å" />

'''Crystal structure of the Salmonella SpvB ATR Domain in complex with NADH'''<br />

==Overview==

Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB, protein for intracellular growth and systemic virulence. SpvB covalently, modifies actin, causing cytoskeletal disruption and apoptosis. We report, here the crystal structure of the catalytic domain of SpvB, and we show by, mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting, its ATPase activity. We also describe two crystal structures of, SpvB-modified, polymerization-deficient actin. These structures reveal, that ADP-ribosylation does not lead to dramatic conformational changes in, actin, suggesting a model in which this large family of toxins inhibits, actin polymerization primarily through steric disruption of intrafilament, contacts.

Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB protein for intracellular growth and systemic virulence. SpvB covalently modifies actin, causing cytoskeletal disruption and apoptosis. We report here the crystal structure of the catalytic domain of SpvB, and we show by mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting its ATPase activity. We also describe two crystal structures of SpvB-modified, polymerization-deficient actin. These structures reveal that ADP-ribosylation does not lead to dramatic conformational changes in actin, suggesting a model in which this large family of toxins inhibits actin polymerization primarily through steric disruption of intrafilament contacts.

==About this Structure==

2GWL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2GWL OCA].

2GWL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWL OCA].

==Reference==

Line 13:

[[Category: Salmonella typhimurium]]

[[Category: Single protein]]

[[Category: Margarit, S.M.]]

[[Category: Margarit, S M.]]

[[Category: Stebbins, C.E.]]

[[Category: Stebbins, C E.]]

[[Category: NAD]]

[[Category: adp-ribosyltransferase]]

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[[Category: spvb]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 11:25:~~58 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:36:05 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2gwl.gif|left|200px]]<br /><applet load="2gwl" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2gwl.gif|left|200px]]<br /><applet load="2gwl" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2gwl, resolution 1.900&Aring;" />
 '''Crystal structure of the Salmonella SpvB ATR Domain in complex with NADH'''<br />
 ==Overview==
-Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB, protein for intracellular growth and systemic virulence. SpvB covalently, modifies actin, causing cytoskeletal disruption and apoptosis. We report, here the crystal structure of the catalytic domain of SpvB, and we show by, mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting, its ATPase activity. We also describe two crystal structures of, SpvB-modified, polymerization-deficient actin. These structures reveal, that ADP-ribosylation does not lead to dramatic conformational changes in, actin, suggesting a model in which this large family of toxins inhibits, actin polymerization primarily through steric disruption of intrafilament, contacts.
+Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB protein for intracellular growth and systemic virulence. SpvB covalently modifies actin, causing cytoskeletal disruption and apoptosis. We report here the crystal structure of the catalytic domain of SpvB, and we show by mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting its ATPase activity. We also describe two crystal structures of SpvB-modified, polymerization-deficient actin. These structures reveal that ADP-ribosylation does not lead to dramatic conformational changes in actin, suggesting a model in which this large family of toxins inhibits actin polymerization primarily through steric disruption of intrafilament contacts.
 ==About this Structure==
-GWL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GWL OCA].
+GWL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWL OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Salmonella typhimurium]]
 [[Category: Single protein]]
-[[Category: Margarit, S.M.]]
+[[Category: Margarit, S M.]]
-[[Category: Stebbins, C.E.]]
+[[Category: Stebbins, C E.]]
 [[Category: NAD]]
 [[Category: adp-ribosyltransferase]]
@@ Line 20: / Line 20: @@
 [[Category: spvb]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:25:58 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:36:05 2008''