2hbl: Difference between revisions

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[[Image:2hbl.gif|left|200px]]<br /><applet load="2hbl" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2hbl.gif|left|200px]]<br /><applet load="2hbl" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2hbl, resolution 2.300Å" />

'''Structure of the yeast nuclear exosome component, Rrp6p, reveals an interplay between the active site and the HRDC domain; Protein in complex with Mn, Zn, and AMP'''<br />

==Overview==

The multisubunit eukaryotic exosome is an essential RNA processing and, degradation machine. In its nuclear form, the exosome associates with the, auxiliary factor Rrp6p, which participates in both RNA processing and, degradation reactions. The crystal structure of Saccharomyces cerevisiae, Rrp6p displays a conserved RNase D core with a flanking HRDC (helicase and, RNase D C-terminal) domain in an unusual conformation shown to be, important for the processing function of the enzyme. Complexes with AMP, and UMP, the products of the RNA degradation process, reveal how the, protein specifically recognizes ribonucleotides and their bases. Finally, in vivo mutational studies show the importance of the domain contacts for, the processing function of Rrp6p and highlight fundamental differences, between the protein and its prokaryotic RNase D counterparts.

The multisubunit eukaryotic exosome is an essential RNA processing and degradation machine. In its nuclear form, the exosome associates with the auxiliary factor Rrp6p, which participates in both RNA processing and degradation reactions. The crystal structure of Saccharomyces cerevisiae Rrp6p displays a conserved RNase D core with a flanking HRDC (helicase and RNase D C-terminal) domain in an unusual conformation shown to be important for the processing function of the enzyme. Complexes with AMP and UMP, the products of the RNA degradation process, reveal how the protein specifically recognizes ribonucleotides and their bases. Finally, in vivo mutational studies show the importance of the domain contacts for the processing function of Rrp6p and highlight fundamental differences between the protein and its prokaryotic RNase D counterparts.

==About this Structure==

2HBL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN, MN and AMP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2HBL OCA].

2HBL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=AMP:'>AMP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HBL OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Assenholt, J.]]

[[Category: Brodersen, D.E.]]

[[Category: Brodersen, D E.]]

[[Category: Jensen, T.H.]]

[[Category: Jensen, T H.]]

[[Category: Jonstrup, A.T.]]

[[Category: Jonstrup, A T.]]

[[Category: Midtgaard, S.F.]]

[[Category: Midtgaard, S F.]]

[[Category: Van, L.B.]]

[[Category: Van, L B.]]

[[Category: AMP]]

[[Category: MN]]

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[[Category: rna surveillance]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 11:39:~~40 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:40:11 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2hbl.gif|left|200px]]<br /><applet load="2hbl" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2hbl.gif|left|200px]]<br /><applet load="2hbl" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2hbl, resolution 2.300&Aring;" />
 '''Structure of the yeast nuclear exosome component, Rrp6p, reveals an interplay between the active site and the HRDC domain; Protein in complex with Mn, Zn, and AMP'''<br />
 ==Overview==
-The multisubunit eukaryotic exosome is an essential RNA processing and, degradation machine. In its nuclear form, the exosome associates with the, auxiliary factor Rrp6p, which participates in both RNA processing and, degradation reactions. The crystal structure of Saccharomyces cerevisiae, Rrp6p displays a conserved RNase D core with a flanking HRDC (helicase and, RNase D C-terminal) domain in an unusual conformation shown to be, important for the processing function of the enzyme. Complexes with AMP, and UMP, the products of the RNA degradation process, reveal how the, protein specifically recognizes ribonucleotides and their bases. Finally, in vivo mutational studies show the importance of the domain contacts for, the processing function of Rrp6p and highlight fundamental differences, between the protein and its prokaryotic RNase D counterparts.
+The multisubunit eukaryotic exosome is an essential RNA processing and degradation machine. In its nuclear form, the exosome associates with the auxiliary factor Rrp6p, which participates in both RNA processing and degradation reactions. The crystal structure of Saccharomyces cerevisiae Rrp6p displays a conserved RNase D core with a flanking HRDC (helicase and RNase D C-terminal) domain in an unusual conformation shown to be important for the processing function of the enzyme. Complexes with AMP and UMP, the products of the RNA degradation process, reveal how the protein specifically recognizes ribonucleotides and their bases. Finally, in vivo mutational studies show the importance of the domain contacts for the processing function of Rrp6p and highlight fundamental differences between the protein and its prokaryotic RNase D counterparts.
 ==About this Structure==
-HBL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN, MN and AMP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HBL OCA].
+HBL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=AMP:'>AMP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HBL OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Assenholt, J.]]
-[[Category: Brodersen, D.E.]]
+[[Category: Brodersen, D E.]]
-[[Category: Jensen, T.H.]]
+[[Category: Jensen, T H.]]
-[[Category: Jonstrup, A.T.]]
+[[Category: Jonstrup, A T.]]
-[[Category: Midtgaard, S.F.]]
+[[Category: Midtgaard, S F.]]
-[[Category: Van, L.B.]]
+[[Category: Van, L B.]]
 [[Category: AMP]]
 [[Category: MN]]
@@ Line 27: / Line 27: @@
 [[Category: rna surveillance]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:39:40 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:40:11 2008''