2hdc: Difference between revisions

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[[Image:2hdc.gif|left|200px]]<br /><applet load="2hdc" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2hdc.gif|left|200px]]<br /><applet load="2hdc" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2hdc" />

'''STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DNA COMPLEX'''<br />

==Overview==

Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR, techniques, we have obtained the solution structure and backbone dynamics, of Genesis complexed with a 17 base-pair DNA. Our results indicate that, both the local folding and dynamic properties of Genesis are perturbed, when it binds to the DNA site. Our data show that a conserved flexible, amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex, and a short helix is induced by Genesis-DNA interactions. Our data, indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not, required in forming the stable Genesis-DNA complex.

Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.

==About this Structure==

2HDC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2HDC OCA].

2HDC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HDC OCA].

==Reference==

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[[Category: winged helix protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 11:41:~~12 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:40:40 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2hdc.gif|left|200px]]<br /><applet load="2hdc" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2hdc.gif|left|200px]]<br /><applet load="2hdc" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2hdc" />
 '''STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DNA COMPLEX'''<br />
 ==Overview==
-Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR, techniques, we have obtained the solution structure and backbone dynamics, of Genesis complexed with a 17 base-pair DNA. Our results indicate that, both the local folding and dynamic properties of Genesis are perturbed, when it binds to the DNA site. Our data show that a conserved flexible, amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex, and a short helix is induced by Genesis-DNA interactions. Our data, indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not, required in forming the stable Genesis-DNA complex.
+Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.
 ==About this Structure==
-HDC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HDC OCA].
+HDC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HDC OCA].
 ==Reference==
@@ Line 23: / Line 23: @@
 [[Category: winged helix protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:41:12 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:40:40 2008''