2hl6: Difference between revisions

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[[Image:2hl6.gif|left|200px]]<br /><applet load="2hl6" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2hl6.gif|left|200px]]<br /><applet load="2hl6" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2hl6, resolution 1.550Å" />

'''Structure of homologously expressed Ferrulate esterase of Aspergillus niger in complex with CAPS'''<br />

==Overview==

The thermal stability of four molecular forms (native, refolded, glycosylated, non-glycosylated) of feruloyl esterase A (FAEA) was studied., From the most to the least thermo-resistant, the four molecular species, ranked as follows: (i) glycosylated form produced native, (ii), non-glycosylated form produced native, (iii) non-glycosylated form, produced as inclusion bodies and refolded, and (iv) glycosylated form, produced native chemically denatured and then refolded. On the basis of, these results and of crystal structure data, we discuss the respective, importance of protein folding and glycosylation in the thermal stability, of recombinant FAEA.

The thermal stability of four molecular forms (native, refolded, glycosylated, non-glycosylated) of feruloyl esterase A (FAEA) was studied. From the most to the least thermo-resistant, the four molecular species ranked as follows: (i) glycosylated form produced native, (ii) non-glycosylated form produced native, (iii) non-glycosylated form produced as inclusion bodies and refolded, and (iv) glycosylated form produced native chemically denatured and then refolded. On the basis of these results and of crystal structure data, we discuss the respective importance of protein folding and glycosylation in the thermal stability of recombinant FAEA.

==About this Structure==

2HL6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with SO4, EDO and CXS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Feruloyl_esterase Feruloyl esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.73 3.1.1.73] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2HL6 OCA].

2HL6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=CXS:'>CXS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Feruloyl_esterase Feruloyl esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.73 3.1.1.73] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HL6 OCA].

==Reference==

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[[Category: glycosylated]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 11:49:~~49 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:43:03 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2hl6.gif|left|200px]]<br /><applet load="2hl6" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2hl6.gif|left|200px]]<br /><applet load="2hl6" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2hl6, resolution 1.550&Aring;" />
 '''Structure of homologously expressed Ferrulate esterase of Aspergillus niger in complex with CAPS'''<br />
 ==Overview==
-The thermal stability of four molecular forms (native, refolded, glycosylated, non-glycosylated) of feruloyl esterase A (FAEA) was studied., From the most to the least thermo-resistant, the four molecular species, ranked as follows: (i) glycosylated form produced native, (ii), non-glycosylated form produced native, (iii) non-glycosylated form, produced as inclusion bodies and refolded, and (iv) glycosylated form, produced native chemically denatured and then refolded. On the basis of, these results and of crystal structure data, we discuss the respective, importance of protein folding and glycosylation in the thermal stability, of recombinant FAEA.
+The thermal stability of four molecular forms (native, refolded, glycosylated, non-glycosylated) of feruloyl esterase A (FAEA) was studied. From the most to the least thermo-resistant, the four molecular species ranked as follows: (i) glycosylated form produced native, (ii) non-glycosylated form produced native, (iii) non-glycosylated form produced as inclusion bodies and refolded, and (iv) glycosylated form produced native chemically denatured and then refolded. On the basis of these results and of crystal structure data, we discuss the respective importance of protein folding and glycosylation in the thermal stability of recombinant FAEA.
 ==About this Structure==
-HL6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with SO4, EDO and CXS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Feruloyl_esterase Feruloyl esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.73 3.1.1.73] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HL6 OCA].
+HL6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=CXS:'>CXS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Feruloyl_esterase Feruloyl esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.73 3.1.1.73] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HL6 OCA].
 ==Reference==
@@ Line 25: / Line 25: @@
 [[Category: glycosylated]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:49:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:43:03 2008''