2hrv: Difference between revisions

[[Image:2hrv.jpg|left|200px]]<br /><applet load="2hrv" size="350" color="white" frame="true" align="right" spinBox="true"  

The crystal structure of the 2A proteinase from human rhinovirus serotype 2 (HRV2-2A(pro)) has been solved to 1.95 A resolution. The structure has an unusual, although chymotrypsin-related, fold comprising a unique four-stranded beta sheet as the N-terminal domain and a six-stranded beta barrel as the C-terminal domain. A tightly bound zinc ion, essential for the stability of HRV2-2A(pro), is tetrahedrally coordinated by three cysteine sulfurs and one histidine nitrogen. The active site consists of a catalytic triad formed by His18, Asp35 and Cys106. Asp35 is additionally involved in an extensive hydrogen-bonding network. Modelling studies reveal a substrate-induced fit that explains the specificity of the subsites S4, S2, S1 and S1'. The structure of HRV2-2A(pro) suggests the mechanism of the cis cleavage and its release from the polyprotein.

2HRV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_rhinovirus_2 Human rhinovirus 2] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Picornain_2A Picornain 2A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.29 3.4.22.29] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HRV OCA].  

[[Category: Cherney, M M.]]

[[Category: James, M N.G.]]

[[Category: Liebig, H D.]]

[[Category: Petersen, J F.W.]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:45:03 2008''