2htn: Difference between revisions
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New page: left|200px<br /><applet load="2htn" size="450" color="white" frame="true" align="right" spinBox="true" caption="2htn, resolution 2.50Å" /> '''E. coli bacterioferr... |
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[[Image:2htn.gif|left|200px]]<br /><applet load="2htn" size=" | [[Image:2htn.gif|left|200px]]<br /><applet load="2htn" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2htn, resolution 2.50Å" /> | caption="2htn, resolution 2.50Å" /> | ||
'''E. coli bacterioferritin in its as-isolated form'''<br /> | '''E. coli bacterioferritin in its as-isolated form'''<br /> | ||
==Overview== | ==Overview== | ||
Escherichia coli bacterioferritin was serendipitously crystallized in a | Escherichia coli bacterioferritin was serendipitously crystallized in a novel cubic crystal form and its structure could be determined to 2.5 A resolution despite a high degree of merohedral twinning. This is the first report of crystallographic data on 'as-isolated' E. coli bacterioferritin. The ferroxidase active site contains positive difference density consistent with two metal ions that had co-purified with the protein. X-ray fluorescence studies suggest that the metal composition is different from that of previous structures and is a mix of zinc and native iron ions. The ferroxidase-centre configuration displays a similar flexibility as previously noted for other bacterioferritins. | ||
==About this Structure== | ==About this Structure== | ||
2HTN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with FE and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] Full crystallographic information is available from [http:// | 2HTN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=FE:'>FE</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HTN OCA]. | ||
==Reference== | ==Reference== | ||
Fortuitous structure determination of 'as-isolated' Escherichia coli bacterioferritin in a novel crystal form., van Eerde A, Wolterink-van Loo S, van der Oost J, Dijkstra BW, Acta | Fortuitous structure determination of 'as-isolated' Escherichia coli bacterioferritin in a novel crystal form., van Eerde A, Wolterink-van Loo S, van der Oost J, Dijkstra BW, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1061-6. Epub 2006 Oct 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17077480 17077480] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Ferroxidase]] | [[Category: Ferroxidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dijkstra, B | [[Category: Dijkstra, B W.]] | ||
[[Category: Eerde, A | [[Category: Eerde, A van.]] | ||
[[Category: Loo, S | [[Category: Loo, S Wolterink-Van.]] | ||
[[Category: Oost, J | [[Category: Oost, J Van Der.]] | ||
[[Category: FE]] | [[Category: FE]] | ||
[[Category: HEM]] | [[Category: HEM]] | ||
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[[Category: protein shell]] | [[Category: protein shell]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:45:35 2008'' | ||
