2hvr: Difference between revisions
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New page: left|200px<br /><applet load="2hvr" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hvr, resolution 2.450Å" /> '''Structure of T4 RNA... |
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[[Image:2hvr.gif|left|200px]]<br /><applet load="2hvr" size=" | [[Image:2hvr.gif|left|200px]]<br /><applet load="2hvr" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2hvr, resolution 2.450Å" /> | caption="2hvr, resolution 2.450Å" /> | ||
'''Structure of T4 RNA Ligase 2 with Nicked 5'-Adenylated nucleic acid duplex containing a 3'-deoxyribonucleotide at the nick'''<br /> | '''Structure of T4 RNA Ligase 2 with Nicked 5'-Adenylated nucleic acid duplex containing a 3'-deoxyribonucleotide at the nick'''<br /> | ||
==Overview== | ==Overview== | ||
T4 RNA ligase 2 (Rnl2) and kinetoplastid RNA editing ligases exemplify a | T4 RNA ligase 2 (Rnl2) and kinetoplastid RNA editing ligases exemplify a family of RNA repair enzymes that seal 3'OH/5'PO(4) nicks in duplex RNAs via ligase adenylylation (step 1), AMP transfer to the nick 5'PO(4) (step 2), and attack by the nick 3'OH on the 5'-adenylylated strand to form a phosphodiester (step 3). Crystal structures are reported for Rnl2 at discrete steps along this pathway: the covalent Rnl2-AMP intermediate; Rnl2 bound to an adenylylated nicked duplex, captured immediately following step 2; and Rnl2 at an adenylylated nick in a state poised for step 3. These structures illuminate the stereochemistry of nucleotidyl transfer and reveal how remodeling of active-site contacts and conformational changes propel the ligation reaction forward. Mutational analysis and comparison of nick-bound structures of Rnl2 and human DNA ligase I highlight common and divergent themes of substrate recognition that can explain their specialization for RNA versus DNA repair. | ||
==About this Structure== | ==About this Structure== | ||
2HVR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with BTB as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 2HVR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=BTB:'>BTB</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HVR OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Bacteriophage t4]] | [[Category: Bacteriophage t4]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Lima, C | [[Category: Lima, C D.]] | ||
[[Category: Nandakumar, J.]] | [[Category: Nandakumar, J.]] | ||
[[Category: BTB]] | [[Category: BTB]] | ||
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[[Category: t4]] | [[Category: t4]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:46:16 2008'' | ||
