2hzv: Difference between revisions
New page: left|200px<br /><applet load="2hzv" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hzv, resolution 3.10Å" /> '''NikR-operator DNA co... |
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[[Image:2hzv.gif|left|200px]]<br /><applet load="2hzv" size=" | [[Image:2hzv.gif|left|200px]]<br /><applet load="2hzv" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2hzv, resolution 3.10Å" /> | caption="2hzv, resolution 3.10Å" /> | ||
'''NikR-operator DNA complex'''<br /> | '''NikR-operator DNA complex'''<br /> | ||
==Overview== | ==Overview== | ||
Metal ion homeostasis is critical to the survival of all cells. Regulation | Metal ion homeostasis is critical to the survival of all cells. Regulation of nickel concentrations in Escherichia coli is mediated by the NikR repressor via nickel-induced transcriptional repression of the nickel ABC-type transporter, NikABCDE. Here, we report two crystal structures of nickel-activated E. coli NikR, the isolated repressor at 2.1 A resolution and in a complex with its operator DNA sequence from the nik promoter at 3.1 A resolution. Along with the previously published structure of apo-NikR, these structures allow us to evaluate functional proposals for how metal ions activate NikR, delineate the drastic conformational changes required for operator recognition, and describe the formation of a second metal-binding site in the presence of DNA. They also provide a rare set of structural views of a ligand-responsive transcription factor in the unbound, ligand-induced, and DNA-bound states, establishing a model system for the study of ligand-mediated effects on transcription factor function. | ||
==About this Structure== | ==About this Structure== | ||
2HZV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with NI and K as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2HZV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=NI:'>NI</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Drennan, C | [[Category: Drennan, C L.]] | ||
[[Category: Schreiter, E | [[Category: Schreiter, E R.]] | ||
[[Category: K]] | [[Category: K]] | ||
[[Category: NI]] | [[Category: NI]] | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:47:33 2008'' | ||
Revision as of 15:47, 21 February 2008
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NikR-operator DNA complex
Overview
Metal ion homeostasis is critical to the survival of all cells. Regulation of nickel concentrations in Escherichia coli is mediated by the NikR repressor via nickel-induced transcriptional repression of the nickel ABC-type transporter, NikABCDE. Here, we report two crystal structures of nickel-activated E. coli NikR, the isolated repressor at 2.1 A resolution and in a complex with its operator DNA sequence from the nik promoter at 3.1 A resolution. Along with the previously published structure of apo-NikR, these structures allow us to evaluate functional proposals for how metal ions activate NikR, delineate the drastic conformational changes required for operator recognition, and describe the formation of a second metal-binding site in the presence of DNA. They also provide a rare set of structural views of a ligand-responsive transcription factor in the unbound, ligand-induced, and DNA-bound states, establishing a model system for the study of ligand-mediated effects on transcription factor function.
About this Structure
2HZV is a Single protein structure of sequence from Escherichia coli with NI and K as ligands. Full crystallographic information is available from OCA.
Reference
NikR-operator complex structure and the mechanism of repressor activation by metal ions., Schreiter ER, Wang SC, Zamble DB, Drennan CL, Proc Natl Acad Sci U S A. 2006 Sep 12;103(37):13676-81. Epub 2006 Aug 31. PMID:16945905
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