2i2t: Difference between revisions

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[[Image:2i2t.gif|left|200px]]<br /><applet load="2i2t" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2i2t.gif|left|200px]]<br /><applet load="2i2t" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2i2t, resolution 3.220Å" />

'''Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. This file contains the 50s subunit of one 70s ribosome. The entire crystal structure contains two 70s ribosomes and is described in remark 400.'''<br />

==Overview==

Protein synthesis requires the accurate positioning of mRNA and tRNA in, the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal, structures of the intact bacterial ribosome from Escherichia coli in a, complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-A, resolution, these structures reveal rearrangements in the intact ribosome, that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of, the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to, lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.

Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-A resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.

==About this Structure==

2I2T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2I2T OCA].

2I2T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2T OCA].

==Reference==

Line 14:

[[Category: Protein complex]]

[[Category: Berk, V.]]

[[Category: Cate, J.H.D.]]

[[Category: Cate, J H.D.]]

[[Category: Pai, R.D.]]

[[Category: Pai, R D.]]

[[Category: Zhang, W.]]

[[Category: MG]]

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[[Category: ribosome]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 12:07:~~13 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:48:26 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2i2t.gif|left|200px]]<br /><applet load="2i2t" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2i2t.gif|left|200px]]<br /><applet load="2i2t" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2i2t, resolution 3.220&Aring;" />
 '''Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. This file contains the 50s subunit of one 70s ribosome. The entire crystal structure contains two 70s ribosomes and is described in remark 400.'''<br />
 ==Overview==
-Protein synthesis requires the accurate positioning of mRNA and tRNA in, the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal, structures of the intact bacterial ribosome from Escherichia coli in a, complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-A, resolution, these structures reveal rearrangements in the intact ribosome, that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of, the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to, lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.
+Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-A resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.
 ==About this Structure==
-I2T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2I2T OCA].
+I2T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2T OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Protein complex]]
 [[Category: Berk, V.]]
-[[Category: Cate, J.H.D.]]
+[[Category: Cate, J H.D.]]
-[[Category: Pai, R.D.]]
+[[Category: Pai, R D.]]
 [[Category: Zhang, W.]]
 [[Category: MG]]
@@ Line 22: / Line 22: @@
 [[Category: ribosome]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:07:13 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:48:26 2008''