2j5p: Difference between revisions
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New page: left|200px<br /><applet load="2j5p" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j5p" /> '''E. COLI FTSK GAMMA DOMAIN'''<br /> ==Overvi... |
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[[Image:2j5p.gif|left|200px]]<br /><applet load="2j5p" size=" | [[Image:2j5p.gif|left|200px]]<br /><applet load="2j5p" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2j5p" /> | caption="2j5p" /> | ||
'''E. COLI FTSK GAMMA DOMAIN'''<br /> | '''E. COLI FTSK GAMMA DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
The bacterial septum-located DNA translocase FtsK coordinates circular | The bacterial septum-located DNA translocase FtsK coordinates circular chromosome segregation with cell division. Rapid translocation of DNA by FtsK is directed by 8-base-pair DNA motifs (KOPS), so that newly replicated termini are brought together at the developing septum, thereby facilitating completion of chromosome segregation. Translocase functions reside in three domains, alpha, beta and gamma. FtsKalphabeta are necessary and sufficient for ATP hydrolysis-dependent DNA translocation, which is modulated by FtsKgamma through its interaction with KOPS. By solving the FtsKgamma structure by NMR, we show that gamma is a winged-helix domain. NMR chemical shift mapping localizes the DNA-binding site on the gamma domain. Mutated proteins with substitutions in the FtsKgamma DNA-recognition helix are impaired in DNA binding and KOPS recognition, yet remain competent in DNA translocation and XerCD-dif site-specific recombination, which facilitates the late stages of chromosome segregation. | ||
==About this Structure== | ==About this Structure== | ||
2J5P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | 2J5P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5P OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Allen, M | [[Category: Allen, M D.]] | ||
[[Category: Arciszewska, L.]] | [[Category: Arciszewska, L.]] | ||
[[Category: Baker, R.]] | [[Category: Baker, R.]] | ||
[[Category: Bekker, C | [[Category: Bekker, C De.]] | ||
[[Category: Bycroft, M.]] | [[Category: Bycroft, M.]] | ||
[[Category: Freund, S | [[Category: Freund, S M.]] | ||
[[Category: Lowe, J.]] | [[Category: Lowe, J.]] | ||
[[Category: Sherratt, D | [[Category: Sherratt, D J.]] | ||
[[Category: Sivanathan, V.]] | [[Category: Sivanathan, V.]] | ||
[[Category: bacterial cell division]] | [[Category: bacterial cell division]] | ||
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[[Category: xercd]] | [[Category: xercd]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:59:28 2008'' | ||
