2lkf: Difference between revisions

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[[Image:2lkf.jpg|left|200px]]<br /><applet load="2lkf" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2lkf.jpg|left|200px]]<br /><applet load="2lkf" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2lkf, resolution 2.5Å" />

'''LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS'''<br />

==Overview==

Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are, self-assembling, channel-forming proteins related in sequence and, function. In the alpha-hemolysin heptamer, the channel-forming, beta-strands and the amino latch make long excursions from the protomer, core. Here we report the crystal structure of the water soluble form of, LukF. In the LukF structure the channel-forming region folds into an, amphipathic, three-strand beta-sheet and the amino latch forms a, beta-strand extending a central beta-sheet. The LukF structure illustrates, how a channel-forming toxin masks protein-protein and protein-membrane, interfaces prior to cell binding and assembly, and together with the, alpha-hemolysin heptamer structure, they define the end points on the, pathway of toxin assembly.

Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are self-assembling, channel-forming proteins related in sequence and function. In the alpha-hemolysin heptamer, the channel-forming beta-strands and the amino latch make long excursions from the protomer core. Here we report the crystal structure of the water soluble form of LukF. In the LukF structure the channel-forming region folds into an amphipathic, three-strand beta-sheet and the amino latch forms a beta-strand extending a central beta-sheet. The LukF structure illustrates how a channel-forming toxin masks protein-protein and protein-membrane interfaces prior to cell binding and assembly, and together with the alpha-hemolysin heptamer structure, they define the end points on the pathway of toxin assembly.

==About this Structure==

2LKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2LKF OCA].

2LKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKF OCA].

==Reference==

Line 13:

[[Category: Single protein]]

[[Category: Staphylococcus aureus]]

[[Category: Gouaux, J.E.]]

[[Category: Gouaux, J E.]]

[[Category: Kamio, Y.]]

[[Category: Nariya, H.]]

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[[Category: pore-forming toxin]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 12:42:~~22 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:07:15 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2lkf.jpg|left|200px]]<br /><applet load="2lkf" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2lkf.jpg|left|200px]]<br /><applet load="2lkf" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2lkf, resolution 2.5&Aring;" />
 '''LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS'''<br />
 ==Overview==
-Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are, self-assembling, channel-forming proteins related in sequence and, function. In the alpha-hemolysin heptamer, the channel-forming, beta-strands and the amino latch make long excursions from the protomer, core. Here we report the crystal structure of the water soluble form of, LukF. In the LukF structure the channel-forming region folds into an, amphipathic, three-strand beta-sheet and the amino latch forms a, beta-strand extending a central beta-sheet. The LukF structure illustrates, how a channel-forming toxin masks protein-protein and protein-membrane, interfaces prior to cell binding and assembly, and together with the, alpha-hemolysin heptamer structure, they define the end points on the, pathway of toxin assembly.
+Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are self-assembling, channel-forming proteins related in sequence and function. In the alpha-hemolysin heptamer, the channel-forming beta-strands and the amino latch make long excursions from the protomer core. Here we report the crystal structure of the water soluble form of LukF. In the LukF structure the channel-forming region folds into an amphipathic, three-strand beta-sheet and the amino latch forms a beta-strand extending a central beta-sheet. The LukF structure illustrates how a channel-forming toxin masks protein-protein and protein-membrane interfaces prior to cell binding and assembly, and together with the alpha-hemolysin heptamer structure, they define the end points on the pathway of toxin assembly.
 ==About this Structure==
-LKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2LKF OCA].
+LKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKF OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Staphylococcus aureus]]
-[[Category: Gouaux, J.E.]]
+[[Category: Gouaux, J E.]]
 [[Category: Kamio, Y.]]
 [[Category: Nariya, H.]]
@@ Line 22: / Line 22: @@
 [[Category: pore-forming toxin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:42:22 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:07:15 2008''