2oig: Difference between revisions

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[[Image:2oig.jpg|left|200px]]<br /><applet load="2oig" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2oig.jpg|left|200px]]<br /><applet load="2oig" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2oig, resolution 3.30Å" />

'''Crystal structure of RS21-C6 core segment and dm5CTP complex'''<br />

==Overview==

RS21-C6, which is highly expressed in all vertebrate genomes and green, plants, is proposed to have nucleoside triphosphate pyrophosphohydrolase, activity. Here, we report the crystal structures of the core fragment of, RS21-C6, named RSCUT, and the complex with the substrate 5-methyl dCTP., The refined structure of RSCUT consists mainly of alpha-helices and shows, formation of a tightly associated tetramer. On the basis of the structure, of the RSCUT-m5dCTP complex and the results of pyrophosphatase activity, assays, several key residues involved in the substrate binding of RS21-C6, have been identified. Tetramer formation is shown to be required for, substrate binding.

RS21-C6, which is highly expressed in all vertebrate genomes and green plants, is proposed to have nucleoside triphosphate pyrophosphohydrolase activity. Here, we report the crystal structures of the core fragment of RS21-C6, named RSCUT, and the complex with the substrate 5-methyl dCTP. The refined structure of RSCUT consists mainly of alpha-helices and shows formation of a tightly associated tetramer. On the basis of the structure of the RSCUT-m5dCTP complex and the results of pyrophosphatase activity assays, several key residues involved in the substrate binding of RS21-C6 have been identified. Tetramer formation is shown to be required for substrate binding.

==About this Structure==

2OIG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with 523 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2OIG OCA].

2OIG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=523:'>523</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OIG OCA].

==Reference==

Crystal ~~Structure~~ of RS21-C6, ~~Involved~~ in ~~Nucleoside Triphosphate Pyrophosphohydrolysis~~., Wu B, Liu Y, Zhao Q, Liao S, Zhang J, Bartlam M, Chen W, Rao Z, J Mol Biol. 2007 Jan 26;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17320107 17320107]

Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis., Wu B, Liu Y, Zhao Q, Liao S, Zhang J, Bartlam M, Chen W, Rao Z, J Mol Biol. 2007 Apr 13;367(5):1405-12. Epub 2007 Jan 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17320107 17320107]

[[Category: Mus musculus]]

[[Category: Single protein]]

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[[Category: substrate complex]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:18:52 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2oig.jpg|left|200px]]<br /><applet load="2oig" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2oig.jpg|left|200px]]<br /><applet load="2oig" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2oig, resolution 3.30&Aring;" />
 '''Crystal structure of RS21-C6 core segment and dm5CTP complex'''<br />
 ==Overview==
-RS21-C6, which is highly expressed in all vertebrate genomes and green, plants, is proposed to have nucleoside triphosphate pyrophosphohydrolase, activity. Here, we report the crystal structures of the core fragment of, RS21-C6, named RSCUT, and the complex with the substrate 5-methyl dCTP., The refined structure of RSCUT consists mainly of alpha-helices and shows, formation of a tightly associated tetramer. On the basis of the structure, of the RSCUT-m5dCTP complex and the results of pyrophosphatase activity, assays, several key residues involved in the substrate binding of RS21-C6, have been identified. Tetramer formation is shown to be required for, substrate binding.
+RS21-C6, which is highly expressed in all vertebrate genomes and green plants, is proposed to have nucleoside triphosphate pyrophosphohydrolase activity. Here, we report the crystal structures of the core fragment of RS21-C6, named RSCUT, and the complex with the substrate 5-methyl dCTP. The refined structure of RSCUT consists mainly of alpha-helices and shows formation of a tightly associated tetramer. On the basis of the structure of the RSCUT-m5dCTP complex and the results of pyrophosphatase activity assays, several key residues involved in the substrate binding of RS21-C6 have been identified. Tetramer formation is shown to be required for substrate binding.
 ==About this Structure==
-OIG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with 523 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OIG OCA].
+OIG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=523:'>523</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OIG OCA].
 ==Reference==
-Crystal Structure of RS21-C6, Involved in Nucleoside Triphosphate Pyrophosphohydrolysis., Wu B, Liu Y, Zhao Q, Liao S, Zhang J, Bartlam M, Chen W, Rao Z, J Mol Biol. 2007 Jan 26;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17320107 17320107]
+Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis., Wu B, Liu Y, Zhao Q, Liao S, Zhang J, Bartlam M, Chen W, Rao Z, J Mol Biol. 2007 Apr 13;367(5):1405-12. Epub 2007 Jan 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17320107 17320107]
 [[Category: Mus musculus]]
 [[Category: Single protein]]
@@ Line 26: / Line 26: @@
 [[Category: substrate complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:12:03 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:18:52 2008''