NS5B: Difference between revisions

Line 27:

----

'''Figure 5'''

'''Figure 5''' shows much of the x-ray crystallography work that has gone into NS5B to date. Each of the forty three structures included in the superposition contain at least one ligand, the ligands range from ions and small molecules to nucleotides and non-nucleoside analogue inhibitors. Positioning the structure in the familiar orientation with the <scene name='NS5B/Ns5b_with_ligands/3'>domains</scene> colored as above, it is obvious that there are two primary areas where ligands are clustering. Several nucleotides, oligonucleotides and non-nucleoside analogue inhibitors can be found in the area right around the <scene name='NS5B/Ns5b_with_ligands/4'>active site</scene>. There is another site that is about 30-35Å from the active site, an <scene name='NS5B/Ns5b_with_ligands/5'>allosteric site</scene>, where again ligands are clustering. There is a rGTP binding site here that seems to activate the enzyme and a nearby site where inhibitors bind and disrupt the activity of the enzyme.

<scene name='NS5B/Ns5b_with_ligands/3'>~~Domains~~</scene>

<scene name='NS5B/Ns5b_with_ligands/4'>~~Active~~ site ~~ligands~~</scene>

The inhibitors that bind near the <scene name='NS5B/Ns5b_with_ligands/4'>active site</scene> are thought to work by disrupting the ''primer grip'' site causing an inability of the enzyme to efficiently hold and extend a growing primer strand. The inhibitors that bind near the <scene name='NS5B/Ns5b_with_ligands/5'>allosteric site</scene> work through a poorly understood mechanism, several explanations for their ability to inhibit the enzyme have been proposed. The common propositions are that the

<scene name='NS5B/Ns5b_with_ligands/5'>~~Allosteric~~ site ~~ligands~~</scene>

----

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 27: / Line 27: @@
 ----
-<applet load='2HAI_ligands.pdb' size='400' frame='true' align='right' caption='Figure 5. NS5B in complex with ligands from deposited structures all superimposed on the 1.58Å resolution 2HAI. Ligands are from 1C2P, 1CSJ, 1GX5, 1GX6, 1NB4, 1NB6, 1NB7, 1HNU, 1NHV, 1OS5, 1QUV, 1YUY, 1YV2, 1YVF, 1YVX, 1YVZ, 1Z4U, 2AWZ, 2AX0, 2AX1, 2BRK, 2BRL, 2D3U, 2D3Z, 2D41, 2GIQ, 2GIR, 2HWH, 2HWI, 2I1R, 2IJN, 2JC0, 2JC1, 3BR9, 3BSA, 3BSC, 3CIZ, 3CJ0, 3CJ2, 3CJ3, 3CJ4, 3CJ5' />
+<applet load='2HAI_ligands.pdb' size='400' frame='true' align='right' caption='Figure 5. NS5B in complex with ligands from deposited structures all superimposed on the 1.58Å resolution 2HAI. Ligands are from: 1C2P, 1CSJ, 1GX5, 1GX6, 1NB4, 1NB6, 1NB7, 1HNU, 1NHV, 1OS5, 1QUV, 1YUY, 1YV2, 1YVF, 1YVX, 1YVZ, 1Z4U, 2AWZ, 2AX0, 2AX1, 2BRK, 2BRL, 2D3U, 2D3Z, 2D41, 2GIQ, 2GIR, 2HWH, 2HWI, 2I1R, 2IJN, 2JC0, 2JC1, 3BR9, 3BSA, 3BSC, 3CIZ, 3CJ0, 3CJ2, 3CJ3, 3CJ4, 3CJ5.' />
-'''Figure 5'''
+'''Figure 5''' shows much of the x-ray crystallography work that has gone into NS5B to date. Each of the forty three structures included in the superposition contain at least one ligand, the ligands range from ions and small molecules to nucleotides and non-nucleoside analogue inhibitors. Positioning the structure in the familiar orientation with the <scene name='NS5B/Ns5b_with_ligands/3'>domains</scene> colored as above, it is obvious that there are two primary areas where ligands are clustering. Several nucleotides, oligonucleotides and non-nucleoside analogue inhibitors can be found in the area right around the <scene name='NS5B/Ns5b_with_ligands/4'>active site</scene>. There is another site that is about 30-35Å from the active site, an <scene name='NS5B/Ns5b_with_ligands/5'>allosteric site</scene>, where again ligands are clustering. There is a rGTP binding site here that seems to activate the enzyme and a nearby site where inhibitors bind and disrupt the activity of the enzyme.
-<scene name='NS5B/Ns5b_with_ligands/3'>Domains</scene>
-<scene name='NS5B/Ns5b_with_ligands/4'>Active site ligands</scene>
+The inhibitors that bind near the <scene name='NS5B/Ns5b_with_ligands/4'>active site</scene> are thought to work by disrupting the ''primer grip'' site causing an inability of the enzyme to efficiently hold and extend a growing primer strand. The inhibitors that bind near the <scene name='NS5B/Ns5b_with_ligands/5'>allosteric site</scene> work through a poorly understood mechanism, several explanations for their ability to inhibit the enzyme have been proposed. The common propositions are that the
-<scene name='NS5B/Ns5b_with_ligands/5'>Allosteric site ligands</scene>
+----