Factor IX: Difference between revisions
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To identify the internal propetide region of the Vitamin K-dependent carboxylase, five mutant carboxylase molecules (F496A, V502A, Q503R, Q503N, and P504Q) were generated identify the residues responsible for homologous substrate propeptide binding. | To identify the internal propetide region of the Vitamin K-dependent carboxylase, five mutant carboxylase molecules (F496A, V502A, Q503R, Q503N, and P504Q) were generated identify the residues responsible for homologous substrate propeptide binding. | ||
Consensus AVFLS2EQANOVLQRRRR | |||
Internal_Propep PFQRTSWVQPLLNDLSPW | |||
F496A PAQRTSWVQPLLNDLSPW | |||
P495V / Q497L VFLRTSWVQPLLNDLSPW | |||
V502A PFQRTSWAQPLLNDLSPW | |||
V502A /P504Q PFQRTSWAQQLLNDLSPW | |||
Q503N PFQRTSWVNPLLNDLSPW | |||
Q503R PFQRTSWVRPLLNDLSPW | |||
P504Q PFQRTSWVQQLLNDLSPW | |||
S510A PFQRTSWVQPLLNDLAPW | |||
W512A PFQRTSWVQPLLNDLSPA | |||
<span style="color:Brown">'''Mutations:'''</span> | <span style="color:Brown">'''Mutations:'''</span> | ||
