G3p: Difference between revisions
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===Initial Observations=== | ===Initial Observations=== | ||
They used NMR spectroscopy to create a structure of the first domains of g3p. Shown is a combination of the 15 most energetically favorable states. Observations of secondary structure are below. | <applet load='1g3p' size='200' frame='true' align='right' caption='D1 and D2 domains of g3p' />They used NMR spectroscopy to create a structure of the first domains of g3p. Shown is a combination of the 15 most energetically favorable states. Observations of secondary structure are below. | ||
In 1998, Lubkowski et al crystallized the first two domains of g3p. They made two overall observations: | In 1998, Lubkowski et al crystallized the first two domains of g3p. They made two overall observations: | ||
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===D1 domain=== | ===D1 domain=== | ||
<applet load='1fgp' size='100' frame='true' align=' | <applet load='1fgp' size='100' frame='true' align='right' caption='Initial structure of D1' />The first structure of g3p entered into the PDB was by Holliger and Riechmann in late 1996-early 1997<ref name="holliger 97"/>. | ||
The D1 domain consists of mostly beta sheets. Both Holliger and Riechmann as well as Lubowski et al noted a <scene name='G3p/N_terminal_alpha_helix/1'>N terminal alpha helix</scene> <ref name="lubkowski"/> in their respective publications<ref name="holliger 97"/><ref name="lubkowski"/>. This aside, five <scene name='G3p/Beta_strands/1'>beta strands</scene> arranged as a barrel-like motif, which participates with two other strands from second domain to make an antiparallel sheet <ref name="lubkowski"/>. Disulfide bonds exist between Cys 7 and Cys 36 (left handed helix) and Cys 46 and Cys 53 (right handed hook) <ref name="lubkowski"/>. | The D1 domain consists of mostly beta sheets. Both Holliger and Riechmann as well as Lubowski et al noted a <scene name='G3p/N_terminal_alpha_helix/1'>N terminal alpha helix</scene> <ref name="lubkowski"/> in their respective publications<ref name="holliger 97"/><ref name="lubkowski"/>. This aside, five <scene name='G3p/Beta_strands/1'>beta strands</scene> arranged as a barrel-like motif, which participates with two other strands from second domain to make an antiparallel sheet <ref name="lubkowski"/>. Disulfide bonds exist between Cys 7 and Cys 36 (left handed helix) and Cys 46 and Cys 53 (right handed hook) <ref name="lubkowski"/>. | ||
