G3p: Difference between revisions

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===Phage Display===

Infection with filamentous phage does not cause host cell lysis or death ~~(Gailus and Rasched)~~.

Infection with filamentous phage does not cause host cell lysis or death <ref name="gailus"> PMID:8026502 </ref>. The N terminus of g3p can be truncated and the peptide of choice can be inserted <ref name="Cabilly"/>. Insertions can also be made between D2 and D3 <ref name="h and r"> PMID: 9032075 </ref>. Specifically, fusions to ther N terminus have no affect on infectivity, between D12 and D3 have 100 fold reduction for peptide insertion, and a 1000 to 100,000 fold for noncovalently interacting peptides <ref name="chatellier"/>.

N terminus can be truncated and the peptide of choice can be inserted (Cabilly)

Insertions can also be ~~done~~ between D2 and D3 (H and R, ~~9032075)~~

Peptides can be fused to CT domain or to the N1 domain, neither areas are near the central area of the horseshoe <ref name="lubkowski"/>.

~~Amino~~ terminus domain is necessary for infection, ~~but~~ full protein does not need to exist for all five particles on the surface ~~(Cabilly)~~.

While the N terminus domain is necessary for infection, the full protein does not need to exist for all five particles on the surface <ref name="cabilly"/>.

~~Fusions to N terminus (no affect on infectivity), between D12 and D3 (100 fold reduction for peptide insertion, and a 1000 to 100,000 fold for noncovalently interacting peptides)(Chatellier et al)~~

==Evolutionarily Related Proteins==

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Lubkowski et al identified the PDZ domain of Human discs large protein (1pdr) as a potentially related protein (Z score = 2.1). This protein is smaller than g3p, and consequently two beta strands in th core of domain share no identity with D2 <ref name="lubkowski"/>.(H and R, 9032075)

~~PTB domain H and R, 9032075)~~

==Links to Available Structures==

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@@ Line 47: / Line 47: @@
 ===Phage Display===
-Infection with filamentous phage does not cause host cell lysis or death (Gailus and Rasched).
+Infection with filamentous phage does not cause host cell lysis or death <ref name="gailus"> PMID:8026502 </ref>. The N terminus of g3p can be truncated and the peptide of choice can be inserted <ref name="Cabilly"/>. Insertions can also be made between D2 and D3 <ref name="h and r"> PMID: 9032075 </ref>.  Specifically, fusions to ther N terminus have no affect on infectivity, between D12 and D3 have 100 fold reduction for peptide insertion, and a 1000 to 100,000 fold for noncovalently interacting peptides <ref name="chatellier"/>.
-N terminus can be truncated and the peptide of choice can be inserted (Cabilly)
-Insertions can also be done between D2 and D3 (H and R, 9032075)
 Peptides can be fused to CT domain or to the N1 domain, neither areas are near the central area of the horseshoe <ref name="lubkowski"/>.
-Amino terminus domain is necessary for infection, but full protein does not need to exist for all five particles on the surface (Cabilly).
+While the N terminus domain is necessary for infection, the full protein does not need to exist for all five particles on the surface <ref name="cabilly"/>.
-Fusions to N terminus (no affect on infectivity), between D12 and D3 (100 fold reduction for peptide insertion, and a 1000 to 100,000 fold for noncovalently interacting peptides)(Chatellier et al)
 ==Evolutionarily Related Proteins==
@@ Line 67: / Line 62: @@
 Lubkowski et al identified the PDZ domain of Human discs large protein (1pdr) as a potentially related protein (Z score = 2.1). This protein is smaller than g3p, and consequently two beta strands in th core of domain share no identity with D2 <ref name="lubkowski"/>.(H and R, 9032075)
-PTB domain H and R, 9032075)
 ==Links to Available Structures==