2sem: Difference between revisions

Line 1:

[[Image:2sem.jpg|left|200px]]<br /><applet load="2sem" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2sem.jpg|left|200px]]<br /><applet load="2sem" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2sem, resolution 2.2Å" />

'''SEM5 SH3 DOMAIN COMPLEXED WITH PEPTOID INHIBITOR'''<br />

==Overview==

Src homology 3 (SH3) and WW protein interaction domains bind specific, proline-rich sequences. However, instead of recognizing critical prolines, on the basis of side chain shape or rigidity, these domains broadly, accepted amide N-substituted residues. Proline is apparently specifically, selected in vivo, despite low complementarity, because it is the only, endogenous N-substituted amino acid. This discriminatory mechanism, explains how these domains achieve specific but low-affinity recognition, a property that is necessary for transient signaling interactions. The, mechanism can be exploited: screening a series of ligands in which key, prolines were replaced by nonnatural N-substituted residues yielded a, ligand that selectively bound the Grb2 SH3 domain with 100 times greater, affinity.

Src homology 3 (SH3) and WW protein interaction domains bind specific proline-rich sequences. However, instead of recognizing critical prolines on the basis of side chain shape or rigidity, these domains broadly accepted amide N-substituted residues. Proline is apparently specifically selected in vivo, despite low complementarity, because it is the only endogenous N-substituted amino acid. This discriminatory mechanism explains how these domains achieve specific but low-affinity recognition, a property that is necessary for transient signaling interactions. The mechanism can be exploited: screening a series of ligands in which key prolines were replaced by nonnatural N-substituted residues yielded a ligand that selectively bound the Grb2 SH3 domain with 100 times greater affinity.

==About this Structure==

2SEM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] with ACE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2SEM OCA].

2SEM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] with <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SEM OCA].

==Reference==

Line 13:

[[Category: Caenorhabditis elegans]]

[[Category: Single protein]]

[[Category: Cohen, F.E.]]

[[Category: Cohen, F E.]]

[[Category: Lim, W.A.]]

[[Category: Lim, W A.]]

[[Category: Nguyen, J.T.]]

[[Category: Nguyen, J T.]]

[[Category: Turck, C.W.]]

[[Category: Turck, C W.]]

[[Category: Zuckermann, R.N.]]

[[Category: Zuckermann, R N.]]

[[Category: ACE]]

[[Category: inhibitors]]

Line 26:

[[Category: signal transduction]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:49:05 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2sem.jpg|left|200px]]<br /><applet load="2sem" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2sem.jpg|left|200px]]<br /><applet load="2sem" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2sem, resolution 2.2&Aring;" />
 '''SEM5 SH3 DOMAIN COMPLEXED WITH PEPTOID INHIBITOR'''<br />
 ==Overview==
-Src homology 3 (SH3) and WW protein interaction domains bind specific, proline-rich sequences. However, instead of recognizing critical prolines, on the basis of side chain shape or rigidity, these domains broadly, accepted amide N-substituted residues. Proline is apparently specifically, selected in vivo, despite low complementarity, because it is the only, endogenous N-substituted amino acid. This discriminatory mechanism, explains how these domains achieve specific but low-affinity recognition, a property that is necessary for transient signaling interactions. The, mechanism can be exploited: screening a series of ligands in which key, prolines were replaced by nonnatural N-substituted residues yielded a, ligand that selectively bound the Grb2 SH3 domain with 100 times greater, affinity.
+Src homology 3 (SH3) and WW protein interaction domains bind specific proline-rich sequences. However, instead of recognizing critical prolines on the basis of side chain shape or rigidity, these domains broadly accepted amide N-substituted residues. Proline is apparently specifically selected in vivo, despite low complementarity, because it is the only endogenous N-substituted amino acid. This discriminatory mechanism explains how these domains achieve specific but low-affinity recognition, a property that is necessary for transient signaling interactions. The mechanism can be exploited: screening a series of ligands in which key prolines were replaced by nonnatural N-substituted residues yielded a ligand that selectively bound the Grb2 SH3 domain with 100 times greater affinity.
 ==About this Structure==
-SEM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] with ACE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2SEM OCA].
+SEM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] with <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SEM OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Caenorhabditis elegans]]
 [[Category: Single protein]]
-[[Category: Cohen, F.E.]]
+[[Category: Cohen, F E.]]
-[[Category: Lim, W.A.]]
+[[Category: Lim, W A.]]
-[[Category: Nguyen, J.T.]]
+[[Category: Nguyen, J T.]]
-[[Category: Turck, C.W.]]
+[[Category: Turck, C W.]]
-[[Category: Zuckermann, R.N.]]
+[[Category: Zuckermann, R N.]]
 [[Category: ACE]]
 [[Category: inhibitors]]
@@ Line 26: / Line 26: @@
 [[Category: signal transduction]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 14:00:59 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:49:05 2008''