2tbd: Difference between revisions

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[[Image:2tbd.jpg|left|200px]]<br /><applet load="2tbd" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2tbd.jpg|left|200px]]<br /><applet load="2tbd" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2tbd" />

'''SV40 T ANTIGEN DNA-BINDING DOMAIN, NMR, 30 STRUCTURES'''<br />

==Overview==

The structure of the domain from simian virus 40 (SV40) large T-antigen, that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has, been determined by nuclear magnetic resonance spectroscopy. The overall, fold, consisting of a central five-stranded antiparallel beta-sheet, flanked by two alpha-helices on one side and one alpha-helix and one, 3(10)-helix on the other, is a new one. Previous mutational analyses have, identified two elements, termed A (approximately 152-155) and B2, (203-207), as essential for origin-specific recognition. These elements, form two closely juxtaposed loops that define a continuous surface on the, protein. The addition of a duplex oligonucleotide containing the origin, recognition pentanucleotide GAGGC induces chemical shift changes and slows, amide proton exchange in resonances from this region, indicating that this, surface directly contacts the DNA.

The structure of the domain from simian virus 40 (SV40) large T-antigen that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has been determined by nuclear magnetic resonance spectroscopy. The overall fold, consisting of a central five-stranded antiparallel beta-sheet flanked by two alpha-helices on one side and one alpha-helix and one 3(10)-helix on the other, is a new one. Previous mutational analyses have identified two elements, termed A (approximately 152-155) and B2 (203-207), as essential for origin-specific recognition. These elements form two closely juxtaposed loops that define a continuous surface on the protein. The addition of a duplex oligonucleotide containing the origin recognition pentanucleotide GAGGC induces chemical shift changes and slows amide proton exchange in resonances from this region, indicating that this surface directly contacts the DNA.

==About this Structure==

2TBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2TBD OCA].

2TBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TBD OCA].

==Reference==

Line 13:

[[Category: Simian virus 40]]

[[Category: Single protein]]

[[Category: Bachovchin, W.W.]]

[[Category: Bachovchin, W W.]]

[[Category: Bullock, P.A.]]

[[Category: Bullock, P A.]]

[[Category: Luo, X.]]

[[Category: Sanford, D.G.]]

[[Category: Sanford, D G.]]

[[Category: acetylation]]

[[Category: dna-binding protein]]

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[[Category: replication]]

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@@ Line 1: / Line 1: @@
-[[Image:2tbd.jpg|left|200px]]<br /><applet load="2tbd" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2tbd.jpg|left|200px]]<br /><applet load="2tbd" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2tbd" />
 '''SV40 T ANTIGEN DNA-BINDING DOMAIN, NMR, 30 STRUCTURES'''<br />
 ==Overview==
-The structure of the domain from simian virus 40 (SV40) large T-antigen, that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has, been determined by nuclear magnetic resonance spectroscopy. The overall, fold, consisting of a central five-stranded antiparallel beta-sheet, flanked by two alpha-helices on one side and one alpha-helix and one, 3(10)-helix on the other, is a new one. Previous mutational analyses have, identified two elements, termed A (approximately 152-155) and B2, (203-207), as essential for origin-specific recognition. These elements, form two closely juxtaposed loops that define a continuous surface on the, protein. The addition of a duplex oligonucleotide containing the origin, recognition pentanucleotide GAGGC induces chemical shift changes and slows, amide proton exchange in resonances from this region, indicating that this, surface directly contacts the DNA.
+The structure of the domain from simian virus 40 (SV40) large T-antigen that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has been determined by nuclear magnetic resonance spectroscopy. The overall fold, consisting of a central five-stranded antiparallel beta-sheet flanked by two alpha-helices on one side and one alpha-helix and one 3(10)-helix on the other, is a new one. Previous mutational analyses have identified two elements, termed A (approximately 152-155) and B2 (203-207), as essential for origin-specific recognition. These elements form two closely juxtaposed loops that define a continuous surface on the protein. The addition of a duplex oligonucleotide containing the origin recognition pentanucleotide GAGGC induces chemical shift changes and slows amide proton exchange in resonances from this region, indicating that this surface directly contacts the DNA.
 ==About this Structure==
-TBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2TBD OCA].
+TBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TBD OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Simian virus 40]]
 [[Category: Single protein]]
-[[Category: Bachovchin, W.W.]]
+[[Category: Bachovchin, W W.]]
-[[Category: Bullock, P.A.]]
+[[Category: Bullock, P A.]]
 [[Category: Luo, X.]]
-[[Category: Sanford, D.G.]]
+[[Category: Sanford, D G.]]
 [[Category: acetylation]]
 [[Category: dna-binding protein]]
@@ Line 24: / Line 24: @@
 [[Category: replication]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 14:03:47 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:49:32 2008''