2tgi: Difference between revisions

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[[Image:2tgi.gif|left|200px]]<br /><applet load="2tgi" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2tgi.gif|left|200px]]<br /><applet load="2tgi" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2tgi, resolution 1.8Å" />

'''CRYSTAL STRUCTURE OF TRANSFORMING GROWTH FACTOR-BETA2: AN UNUSUAL FOLD FOR THE SUPERFAMILY'''<br />

==Overview==

The transforming growth factors-beta (TGF-beta 1 through -beta 5) are a, family of homodimeric cytokines that regulate proliferation and function, in many cell types. Family members have 66 to 80% sequence identity and, nine strictly conserved cysteines. A crystal structure of a member of this, family, TGF-beta 2, has been determined at 2.1 angstrom (A) resolution and, refined to an R factor of 0.172. The monomer lacks a well-defined, hydrophobic core and displays an unusual elongated nonglobular fold with, dimensions of approximately 60 A by 20 A by 15 A. Eight cysteines form, four intrachain disulfide bonds, which are clustered in a core region, forming a network complementary to the network of hydrogen bonds. The, dimer is stabilized by the ninth cysteine, which forms an interchain, disulfide bond, and by two identical hydrophobic interfaces. Sequence, profile analysis of other members of the TGF-beta superfamily, including, the activins, inhibins, and several developmental factors, imply that they, also adopt the TGF-beta fold.

The transforming growth factors-beta (TGF-beta 1 through -beta 5) are a family of homodimeric cytokines that regulate proliferation and function in many cell types. Family members have 66 to 80% sequence identity and nine strictly conserved cysteines. A crystal structure of a member of this family, TGF-beta 2, has been determined at 2.1 angstrom (A) resolution and refined to an R factor of 0.172. The monomer lacks a well-defined hydrophobic core and displays an unusual elongated nonglobular fold with dimensions of approximately 60 A by 20 A by 15 A. Eight cysteines form four intrachain disulfide bonds, which are clustered in a core region forming a network complementary to the network of hydrogen bonds. The dimer is stabilized by the ninth cysteine, which forms an interchain disulfide bond, and by two identical hydrophobic interfaces. Sequence profile analysis of other members of the TGF-beta superfamily, including the activins, inhibins, and several developmental factors, imply that they also adopt the TGF-beta fold.

==About this Structure==

2TGI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2TGI OCA].

2TGI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TGI OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Daopin, S.]]

[[Category: Davies, D.R.]]

[[Category: Davies, D R.]]

[[Category: growth factor]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 14:04:~~35 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:49:40 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2tgi.gif|left|200px]]<br /><applet load="2tgi" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2tgi.gif|left|200px]]<br /><applet load="2tgi" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2tgi, resolution 1.8&Aring;" />
 '''CRYSTAL STRUCTURE OF TRANSFORMING GROWTH FACTOR-BETA2: AN UNUSUAL FOLD FOR THE SUPERFAMILY'''<br />
 ==Overview==
-The transforming growth factors-beta (TGF-beta 1 through -beta 5) are a, family of homodimeric cytokines that regulate proliferation and function, in many cell types. Family members have 66 to 80% sequence identity and, nine strictly conserved cysteines. A crystal structure of a member of this, family, TGF-beta 2, has been determined at 2.1 angstrom (A) resolution and, refined to an R factor of 0.172. The monomer lacks a well-defined, hydrophobic core and displays an unusual elongated nonglobular fold with, dimensions of approximately 60 A by 20 A by 15 A. Eight cysteines form, four intrachain disulfide bonds, which are clustered in a core region, forming a network complementary to the network of hydrogen bonds. The, dimer is stabilized by the ninth cysteine, which forms an interchain, disulfide bond, and by two identical hydrophobic interfaces. Sequence, profile analysis of other members of the TGF-beta superfamily, including, the activins, inhibins, and several developmental factors, imply that they, also adopt the TGF-beta fold.
+The transforming growth factors-beta (TGF-beta 1 through -beta 5) are a family of homodimeric cytokines that regulate proliferation and function in many cell types. Family members have 66 to 80% sequence identity and nine strictly conserved cysteines. A crystal structure of a member of this family, TGF-beta 2, has been determined at 2.1 angstrom (A) resolution and refined to an R factor of 0.172. The monomer lacks a well-defined hydrophobic core and displays an unusual elongated nonglobular fold with dimensions of approximately 60 A by 20 A by 15 A. Eight cysteines form four intrachain disulfide bonds, which are clustered in a core region forming a network complementary to the network of hydrogen bonds. The dimer is stabilized by the ninth cysteine, which forms an interchain disulfide bond, and by two identical hydrophobic interfaces. Sequence profile analysis of other members of the TGF-beta superfamily, including the activins, inhibins, and several developmental factors, imply that they also adopt the TGF-beta fold.
 ==About this Structure==
-TGI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2TGI OCA].
+TGI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TGI OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Daopin, S.]]
-[[Category: Davies, D.R.]]
+[[Category: Davies, D R.]]
 [[Category: growth factor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 14:04:35 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:49:40 2008''