2ush: Difference between revisions
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New page: left|200px<br /><applet load="2ush" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ush, resolution 2.22Å" /> '''5'-NUCLEOTIDASE FROM... |
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[[Image:2ush.jpg|left|200px]]<br /><applet load="2ush" size=" | [[Image:2ush.jpg|left|200px]]<br /><applet load="2ush" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2ush, resolution 2.22Å" /> | caption="2ush, resolution 2.22Å" /> | ||
'''5'-NUCLEOTIDASE FROM E. COLI'''<br /> | '''5'-NUCLEOTIDASE FROM E. COLI'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of 5'-nucleotidase (5'-NT) from E. coli, also known | The crystal structure of 5'-nucleotidase (5'-NT) from E. coli, also known as UDP-sugar hydrolase, has been determined at 1.7 A resolution. Two zinc ions are present in the active site, which is located in a cleft between two domains. The dimetal center and a catalytic Asp-His dyad are the main players in the catalytic mechanism. Structure-based sequence comparisons show that the structure also provides a model for animal 5'-NTs, which together with other ectonucleotidases terminate the action of nucleotides as extracellular signaling substances in the nervous system. | ||
==About this Structure== | ==About this Structure== | ||
2USH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and WO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] Full crystallographic information is available from [http:// | 2USH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=WO4:'>WO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2USH OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: WO4]] | [[Category: WO4]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: hydrolase (phosphoric monoester)]] | [[Category: hydrolase (phosphoric monoester)]] | ||
[[Category: periplasmic protein]] | [[Category: periplasmic protein]] | ||
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[[Category: udp-sugar hydrolase]] | [[Category: udp-sugar hydrolase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:50:15 2008'' | ||
