1icg: Difference between revisions

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[[Image:1icg.gif|left|200px]]<br /><applet load="1icg" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1icg.gif|left|200px]]<br /><applet load="1icg" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1icg, resolution 1.53Å" />

'''STRUCTURE OF THE HYBRID RNA/DNA R-GCUUCGGC-D[F]U IN PRESENCE OF IR(NH3)6+++'''<br />

==Overview==

In the presence of cobalt, rhodium or iridium hexammine salts, the RNA/DNA, hybrid r-GCUUCGGC-d(X)U (with X = F, Cl or Br) crystallizes as a, double-stranded helix with four consecutive G-U and C-U mismatches. The, deoxy chloro- and bromouracil derivatives are isomorphous, space group C2, unit-cell parameters a = 53.80, b = 19.40, c = 50.31 A, beta = 109.9, degrees, with the same infinite helix arrangement in the packing along the, c axis with one extra DNA halogenouracil base included in the stacking., However, the fluorouracil derivative, with unit-cell parameters a = 53.75, b = 19.40, c = 45.84 A, beta = 105.7 degrees, is not isomorphous. The, corresponding extra DNA base d(F)U of the second strand is ejected out of, the helical stack, leading to a shortening of the c axis. The specific, destabilization of the fluorouracil for the duplex building is analyzed in, terms of the polarization effect of the halogen atom attached to the, 3'-terminal base that modulates its interactions.

In the presence of cobalt, rhodium or iridium hexammine salts, the RNA/DNA hybrid r-GCUUCGGC-d(X)U (with X = F, Cl or Br) crystallizes as a double-stranded helix with four consecutive G-U and C-U mismatches. The deoxy chloro- and bromouracil derivatives are isomorphous, space group C2, unit-cell parameters a = 53.80, b = 19.40, c = 50.31 A, beta = 109.9 degrees, with the same infinite helix arrangement in the packing along the c axis with one extra DNA halogenouracil base included in the stacking. However, the fluorouracil derivative, with unit-cell parameters a = 53.75, b = 19.40, c = 45.84 A, beta = 105.7 degrees, is not isomorphous. The corresponding extra DNA base d(F)U of the second strand is ejected out of the helical stack, leading to a shortening of the c axis. The specific destabilization of the fluorouracil for the duplex building is analyzed in terms of the polarization effect of the halogen atom attached to the 3'-terminal base that modulates its interactions.

==About this Structure==

1ICG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CL and IRI as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1ICG OCA].

1ICG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=IRI:'>IRI</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ICG OCA].

==Reference==

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[[Category: rna/dna hybrid]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:10:23 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1icg.gif|left|200px]]<br /><applet load="1icg" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1icg.gif|left|200px]]<br /><applet load="1icg" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1icg, resolution 1.53&Aring;" />
 '''STRUCTURE OF THE HYBRID RNA/DNA R-GCUUCGGC-D[F]U IN PRESENCE OF IR(NH3)6+++'''<br />
 ==Overview==
-In the presence of cobalt, rhodium or iridium hexammine salts, the RNA/DNA, hybrid r-GCUUCGGC-d(X)U (with X = F, Cl or Br) crystallizes as a, double-stranded helix with four consecutive G-U and C-U mismatches. The, deoxy chloro- and bromouracil derivatives are isomorphous, space group C2, unit-cell parameters a = 53.80, b = 19.40, c = 50.31 A, beta = 109.9, degrees, with the same infinite helix arrangement in the packing along the, c axis with one extra DNA halogenouracil base included in the stacking., However, the fluorouracil derivative, with unit-cell parameters a = 53.75, b = 19.40, c = 45.84 A, beta = 105.7 degrees, is not isomorphous. The, corresponding extra DNA base d(F)U of the second strand is ejected out of, the helical stack, leading to a shortening of the c axis. The specific, destabilization of the fluorouracil for the duplex building is analyzed in, terms of the polarization effect of the halogen atom attached to the, 3'-terminal base that modulates its interactions.
+In the presence of cobalt, rhodium or iridium hexammine salts, the RNA/DNA hybrid r-GCUUCGGC-d(X)U (with X = F, Cl or Br) crystallizes as a double-stranded helix with four consecutive G-U and C-U mismatches. The deoxy chloro- and bromouracil derivatives are isomorphous, space group C2, unit-cell parameters a = 53.80, b = 19.40, c = 50.31 A, beta = 109.9 degrees, with the same infinite helix arrangement in the packing along the c axis with one extra DNA halogenouracil base included in the stacking. However, the fluorouracil derivative, with unit-cell parameters a = 53.75, b = 19.40, c = 45.84 A, beta = 105.7 degrees, is not isomorphous. The corresponding extra DNA base d(F)U of the second strand is ejected out of the helical stack, leading to a shortening of the c axis. The specific destabilization of the fluorouracil for the duplex building is analyzed in terms of the polarization effect of the halogen atom attached to the 3'-terminal base that modulates its interactions.
 ==About this Structure==
-ICG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CL and IRI as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ICG OCA].
+ICG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=IRI:'>IRI</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ICG OCA].
 ==Reference==
@@ Line 25: / Line 25: @@
 [[Category: rna/dna hybrid]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 21:46:21 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:10:23 2008''