121d: Difference between revisions

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[[Image:121d.jpg|left|200px]]<br /><applet load="121d" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:121d.jpg|left|200px]]<br /><applet load="121d" size="350" color="white" frame="true" align="right" spinBox="true"

caption="121d, resolution 2.200Å" />

'''MOLECULAR STRUCTURE OF THE A-TRACT DNA DODECAMER D(CGCAAATTTGCG) COMPLEXED WITH THE MINOR GROOVE BINDING DRUG NETROPSIN'''<br />

==Overview==

The molecular structure of the complex between the minor groove binding, drug netropsin and the dodecamer d(CGCAAATTTGCG) has been solved and, refined by X-ray diffraction analysis to an R-factor of 19.8% and 2.2-A, resolution. The drug lies in the narrow minor groove of the B-DNA, fragment, covering five of the six A.T base pairs (from A5.T20 to T9.A16)., The long six A.T base pair tract allows the drug to bind in a position, that optimizes its contacts with the DNA, establishing hydrogen bonds with, O2 of thymines and N3 of adenines. The DNA molecule shows a high propeller, twist only at the A6.T19 step of the A-tract. Two three-centered hydrogen, bonds are observed in the major groove at half of the A-tract.

The molecular structure of the complex between the minor groove binding drug netropsin and the dodecamer d(CGCAAATTTGCG) has been solved and refined by X-ray diffraction analysis to an R-factor of 19.8% and 2.2-A resolution. The drug lies in the narrow minor groove of the B-DNA fragment, covering five of the six A.T base pairs (from A5.T20 to T9.A16). The long six A.T base pair tract allows the drug to bind in a position that optimizes its contacts with the DNA, establishing hydrogen bonds with O2 of thymines and N3 of adenines. The DNA molecule shows a high propeller twist only at the A6.T19 step of the A-tract. Two three-centered hydrogen bonds are observed in the major groove at half of the A-tract.

==About this Structure==

121D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=121D OCA].

121D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NT:'>NT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=121D OCA].

==Reference==

Line 13:

[[Category: Protein complex]]

[[Category: Aymami, J.]]

[[Category: Boom, J.H.Van.]]

[[Category: Boom, J H.Van.]]

[[Category: Coll, M.]]

[[Category: Fita, I.]]

[[Category: Marel, G.A.Van.Der.]]

[[Category: Marel, G A.Van Der.]]

[[Category: Rich, A.]]

[[Category: Tabernero, L.]]

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[[Category: double helix]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sat Nov 24~~ 21:48~~:11 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:37:48 2008''

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@@ Line 1: / Line 1: @@
-[[Image:121d.jpg|left|200px]]<br /><applet load="121d" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:121d.jpg|left|200px]]<br /><applet load="121d" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="121d, resolution 2.200&Aring;" />
 '''MOLECULAR STRUCTURE OF THE A-TRACT DNA DODECAMER D(CGCAAATTTGCG) COMPLEXED WITH THE MINOR GROOVE BINDING DRUG NETROPSIN'''<br />
 ==Overview==
-The molecular structure of the complex between the minor groove binding, drug netropsin and the dodecamer d(CGCAAATTTGCG) has been solved and, refined by X-ray diffraction analysis to an R-factor of 19.8% and 2.2-A, resolution. The drug lies in the narrow minor groove of the B-DNA, fragment, covering five of the six A.T base pairs (from A5.T20 to T9.A16)., The long six A.T base pair tract allows the drug to bind in a position, that optimizes its contacts with the DNA, establishing hydrogen bonds with, O2 of thymines and N3 of adenines. The DNA molecule shows a high propeller, twist only at the A6.T19 step of the A-tract. Two three-centered hydrogen, bonds are observed in the major groove at half of the A-tract.
+The molecular structure of the complex between the minor groove binding drug netropsin and the dodecamer d(CGCAAATTTGCG) has been solved and refined by X-ray diffraction analysis to an R-factor of 19.8% and 2.2-A resolution. The drug lies in the narrow minor groove of the B-DNA fragment, covering five of the six A.T base pairs (from A5.T20 to T9.A16). The long six A.T base pair tract allows the drug to bind in a position that optimizes its contacts with the DNA, establishing hydrogen bonds with O2 of thymines and N3 of adenines. The DNA molecule shows a high propeller twist only at the A6.T19 step of the A-tract. Two three-centered hydrogen bonds are observed in the major groove at half of the A-tract.
 ==About this Structure==
-D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=121D OCA].
+D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NT:'>NT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=121D OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Aymami, J.]]
-[[Category: Boom, J.H.Van.]]
+[[Category: Boom, J H.Van.]]
 [[Category: Coll, M.]]
 [[Category: Fita, I.]]
-[[Category: Marel, G.A.Van.Der.]]
+[[Category: Marel, G A.Van Der.]]
 [[Category: Rich, A.]]
 [[Category: Tabernero, L.]]
@@ Line 25: / Line 25: @@
 [[Category: double helix]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 21:48:11 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:37:48 2008''