2zxe

File:2zxe.png

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2zxe, resolution 2.40Å (default scene)
Ligands:	CLR, K, MF4, MG, NAG, NDG
Related:	1wpg
Structural annotation: Resources: InterPro : Ipr001757, Ipr004014, Ipr005775, Ipr005834, Ipr006068, Ipr006069, Ipr008250, Ipr018303, Ipr000272 Pfam : PF00689, PF00702, PF00122, PF00690, PF02038
Evolutionary conservation: Toggle Conservation Colors: Rows = identical sequences: Further Information: Caveats, Explanation, Complete Results at ConSurfDB
Resources:	FirstGlance, OCA, RCSB, PDBsum
Coordinates:	save as pdb, mmCIF, xml

Crystal structure of the sodium - potassium pump in the E2.2K+.Pi state

Publication Abstract from PubMed

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Sodium-potassium ATPase is an ATP-powered ion pump that establishes concentration gradients for Na(+) and K(+) ions across the plasma membrane in all animal cells by pumping Na(+) from the cytoplasm and K(+) from the extracellular medium. Such gradients are used in many essential processes, notably for generating action potentials. Na(+), K(+)-ATPase is a member of the P-type ATPases, which include sarcoplasmic reticulum Ca(2+)-ATPase and gastric H(+), K(+)-ATPase, among others, and is the target of cardiac glycosides. Here we describe a crystal structure of this important ion pump, from shark rectal glands, consisting of alpha- and beta-subunits and a regulatory FXYD protein, all of which are highly homologous to human ones. The ATPase was fixed in a state analogous to E2.2K(+).P(i), in which the ATPase has a high affinity for K(+) and still binds P(i), as in the first crystal structure of pig kidney enzyme at 3.5 A resolution. Clearly visualized now at 2.4 A resolution are coordination of K(+) and associated water molecules in the transmembrane binding sites and a phosphate analogue (MgF(4)(2-)) in the phosphorylation site. The crystal structure shows that the beta-subunit has a critical role in K(+) binding (although its involvement has previously been suggested) and explains, at least partially, why the homologous Ca(2+)-ATPase counter-transports H(+) rather than K(+), despite the coordinating residues being almost identical.

Crystal structure of the sodium-potassium pump at 2.4 A resolution., Shinoda T, Ogawa H, Cornelius F, Toyoshima C, Nature. 2009 May 21;459(7245):446-50. PMID:19458722

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2ZXE is a 3 chains structure of sequences from Squalus acanthias. Full crystallographic information is available from OCA.

Reference

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