1r3g: Difference between revisions

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[[Image:1r3g.gif|left|200px]]<br /><applet load="1r3g" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1r3g.gif|left|200px]]<br /><applet load="1r3g" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1r3g, resolution 1.16Å" />

'''1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues'''<br />

==Overview==

[structure: see text] Oligonucleotides with a novel, 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized, using a novel protecting group strategy for the guanidinium group. This, modification enhances the binding affinity of oligonucleotides to RNA as, well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE, modified oligonucleotides exhibited exceptional resistance to nuclease, degradation. The crystal structure of a palindromic duplex formed by a DNA, oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A, resolution.

[structure: see text] Oligonucleotides with a novel 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized using a novel protecting group strategy for the guanidinium group. This modification enhances the binding affinity of oligonucleotides to RNA as well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE modified oligonucleotides exhibited exceptional resistance to nuclease degradation. The crystal structure of a palindromic duplex formed by a DNA oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A resolution.

==About this Structure==

1R3G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1R3G OCA].

1R3G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R3G OCA].

==Reference==

Line 15:

[[Category: Lesnik, E.]]

[[Category: Manoharan, M.]]

[[Category: Prakash, T.P.]]

[[Category: Prakash, T P.]]

[[Category: Puschl, A.]]

[[Category: Tereshko, V.]]

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[[Category: dna]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sat Nov 24 22~~:02:~~37 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:46:35 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1r3g.gif|left|200px]]<br /><applet load="1r3g" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1r3g.gif|left|200px]]<br /><applet load="1r3g" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1r3g, resolution 1.16&Aring;" />
 '''1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues'''<br />
 ==Overview==
-[structure: see text] Oligonucleotides with a novel, 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized, using a novel protecting group strategy for the guanidinium group. This, modification enhances the binding affinity of oligonucleotides to RNA as, well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE, modified oligonucleotides exhibited exceptional resistance to nuclease, degradation. The crystal structure of a palindromic duplex formed by a DNA, oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A, resolution.
+[structure: see text] Oligonucleotides with a novel 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized using a novel protecting group strategy for the guanidinium group. This modification enhances the binding affinity of oligonucleotides to RNA as well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE modified oligonucleotides exhibited exceptional resistance to nuclease degradation. The crystal structure of a palindromic duplex formed by a DNA oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A resolution.
 ==About this Structure==
-R3G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R3G OCA].
+R3G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R3G OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Lesnik, E.]]
 [[Category: Manoharan, M.]]
-[[Category: Prakash, T.P.]]
+[[Category: Prakash, T P.]]
 [[Category: Puschl, A.]]
 [[Category: Tereshko, V.]]
@@ Line 21: / Line 21: @@
 [[Category: dna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:02:37 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:46:35 2008''