1mur: Difference between revisions

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[[Image:1mur.gif|left|200px]]<br /><applet load="1mur" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1mur.gif|left|200px]]<br /><applet load="1mur" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1mur, resolution 2.50Å" />

'''TN5 TRANSPOSASE: 20MER OUTSIDE END 2 MN COMPLEX'''<br />

==Overview==

A synaptic complex of Tn5 transposase with an extended outside end DNA, duplex was prepared and crystallized, and its crystal structure was, determined in an effort to reveal the role of metal ions in catalysis. Two, Mn2+ ions bound to the active site when a single nucleotide of donor DNA, was added to the 3' end of the transferred strand. Marked conformational, changes were observed in the DNA bases closest to the active site. The, position of the metal ions and the conformational changes of the DNA, provide insight into the mechanism of hairpin formation and cleavage, and, is consistent with a two-metal model for catalysis.

A synaptic complex of Tn5 transposase with an extended outside end DNA duplex was prepared and crystallized, and its crystal structure was determined in an effort to reveal the role of metal ions in catalysis. Two Mn2+ ions bound to the active site when a single nucleotide of donor DNA was added to the 3' end of the transferred strand. Marked conformational changes were observed in the DNA bases closest to the active site. The position of the metal ions and the conformational changes of the DNA provide insight into the mechanism of hairpin formation and cleavage, and is consistent with a two-metal model for catalysis.

==About this Structure==

1MUR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. This structure ~~superseeds~~ the now removed PDB entry 1L1A. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1MUR OCA].

1MUR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entry 1L1A. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUR OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Goryshin, I.Y.]]

[[Category: Goryshin, I Y.]]

[[Category: Holden, H.M.]]

[[Category: Holden, H M.]]

[[Category: Lovell, S.]]

[[Category: Rayment, I.]]

[[Category: Reznikoff, W.R.]]

[[Category: Reznikoff, W R.]]

[[Category: Thoden, J.B.]]

[[Category: Thoden, J B.]]

[[Category: MN]]

[[Category: dna binding]]

[[Category: transposase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sat Nov 24 22~~:02:~~40 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:59:14 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1mur.gif|left|200px]]<br /><applet load="1mur" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1mur.gif|left|200px]]<br /><applet load="1mur" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1mur, resolution 2.50&Aring;" />
 '''TN5 TRANSPOSASE: 20MER OUTSIDE END 2 MN COMPLEX'''<br />
 ==Overview==
-A synaptic complex of Tn5 transposase with an extended outside end DNA, duplex was prepared and crystallized, and its crystal structure was, determined in an effort to reveal the role of metal ions in catalysis. Two, Mn2+ ions bound to the active site when a single nucleotide of donor DNA, was added to the 3' end of the transferred strand. Marked conformational, changes were observed in the DNA bases closest to the active site. The, position of the metal ions and the conformational changes of the DNA, provide insight into the mechanism of hairpin formation and cleavage, and, is consistent with a two-metal model for catalysis.
+A synaptic complex of Tn5 transposase with an extended outside end DNA duplex was prepared and crystallized, and its crystal structure was determined in an effort to reveal the role of metal ions in catalysis. Two Mn2+ ions bound to the active site when a single nucleotide of donor DNA was added to the 3' end of the transferred strand. Marked conformational changes were observed in the DNA bases closest to the active site. The position of the metal ions and the conformational changes of the DNA provide insight into the mechanism of hairpin formation and cleavage, and is consistent with a two-metal model for catalysis.
 ==About this Structure==
-MUR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 1L1A. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MUR OCA].
+MUR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entry 1L1A. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUR OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Goryshin, I.Y.]]
+[[Category: Goryshin, I Y.]]
-[[Category: Holden, H.M.]]
+[[Category: Holden, H M.]]
 [[Category: Lovell, S.]]
 [[Category: Rayment, I.]]
-[[Category: Reznikoff, W.R.]]
+[[Category: Reznikoff, W R.]]
-[[Category: Thoden, J.B.]]
+[[Category: Thoden, J B.]]
 [[Category: MN]]
 [[Category: dna binding]]
 [[Category: transposase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:02:40 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:59:14 2008''