1r5e: Difference between revisions

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[[Image:1r5e.jpg|left|200px]]<br /><applet load="1r5e" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1r5e.jpg|left|200px]]<br /><applet load="1r5e" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1r5e" />

'''Solution structure of the folded core of Pseudomonas syringae effector protein, AvrPto'''<br />

==Overview==

Pseudomonas syringae pv. tomato, the causative agent of bacterial speck, disease of tomato, uses a type III secretion system (TTSS) to deliver, effector proteins into the host cell. In resistant plants, the bacterial, effector protein AvrPto physically interacts with the host Pto kinase and, elicits antibacterial defense responses. In susceptible plants, which lack, the Pto kinase, AvrPto acts as a virulence factor to promote bacterial, growth. The solution structure of AvrPto reveals a functional core, consisting of a three-helix bundle motif flanked by disordered N- and, C-terminal tails. Residues required for Pto binding lie in a 19 residue, Omega loop. Modeling suggests a hydrophobic patch involving the activation, loop of Pto forms a contact surface with the AvrPto Omega loop and that, helix packing mediates interactions between AvrPto and putative virulence, targets Api2 and Api3. The AvrPto structure has a low stability that may, facilitate chaperone-independent secretion by the TTSS.

Pseudomonas syringae pv. tomato, the causative agent of bacterial speck disease of tomato, uses a type III secretion system (TTSS) to deliver effector proteins into the host cell. In resistant plants, the bacterial effector protein AvrPto physically interacts with the host Pto kinase and elicits antibacterial defense responses. In susceptible plants, which lack the Pto kinase, AvrPto acts as a virulence factor to promote bacterial growth. The solution structure of AvrPto reveals a functional core consisting of a three-helix bundle motif flanked by disordered N- and C-terminal tails. Residues required for Pto binding lie in a 19 residue Omega loop. Modeling suggests a hydrophobic patch involving the activation loop of Pto forms a contact surface with the AvrPto Omega loop and that helix packing mediates interactions between AvrPto and putative virulence targets Api2 and Api3. The AvrPto structure has a low stability that may facilitate chaperone-independent secretion by the TTSS.

==About this Structure==

1R5E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1R5E OCA].

1R5E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5E OCA].

==Reference==

Line 13:

[[Category: Pseudomonas syringae]]

[[Category: Single protein]]

[[Category: Martin, G.B.]]

[[Category: Martin, G B.]]

[[Category: Nicholson, L.K.]]

[[Category: Nicholson, L K.]]

[[Category: Pascuzzi, P.E.]]

[[Category: Pascuzzi, P E.]]

[[Category: Wulf, J.]]

[[Category: omega loop]]

[[Category: three-helix bundle]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sat Nov 24 22~~:10:~~37 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:47:15 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1r5e.jpg|left|200px]]<br /><applet load="1r5e" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1r5e.jpg|left|200px]]<br /><applet load="1r5e" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1r5e" />
 '''Solution structure of the folded core of Pseudomonas syringae effector protein, AvrPto'''<br />
 ==Overview==
-Pseudomonas syringae pv. tomato, the causative agent of bacterial speck, disease of tomato, uses a type III secretion system (TTSS) to deliver, effector proteins into the host cell. In resistant plants, the bacterial, effector protein AvrPto physically interacts with the host Pto kinase and, elicits antibacterial defense responses. In susceptible plants, which lack, the Pto kinase, AvrPto acts as a virulence factor to promote bacterial, growth. The solution structure of AvrPto reveals a functional core, consisting of a three-helix bundle motif flanked by disordered N- and, C-terminal tails. Residues required for Pto binding lie in a 19 residue, Omega loop. Modeling suggests a hydrophobic patch involving the activation, loop of Pto forms a contact surface with the AvrPto Omega loop and that, helix packing mediates interactions between AvrPto and putative virulence, targets Api2 and Api3. The AvrPto structure has a low stability that may, facilitate chaperone-independent secretion by the TTSS.
+Pseudomonas syringae pv. tomato, the causative agent of bacterial speck disease of tomato, uses a type III secretion system (TTSS) to deliver effector proteins into the host cell. In resistant plants, the bacterial effector protein AvrPto physically interacts with the host Pto kinase and elicits antibacterial defense responses. In susceptible plants, which lack the Pto kinase, AvrPto acts as a virulence factor to promote bacterial growth. The solution structure of AvrPto reveals a functional core consisting of a three-helix bundle motif flanked by disordered N- and C-terminal tails. Residues required for Pto binding lie in a 19 residue Omega loop. Modeling suggests a hydrophobic patch involving the activation loop of Pto forms a contact surface with the AvrPto Omega loop and that helix packing mediates interactions between AvrPto and putative virulence targets Api2 and Api3. The AvrPto structure has a low stability that may facilitate chaperone-independent secretion by the TTSS.
 ==About this Structure==
-R5E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R5E OCA].
+R5E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5E OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Pseudomonas syringae]]
 [[Category: Single protein]]
-[[Category: Martin, G.B.]]
+[[Category: Martin, G B.]]
-[[Category: Nicholson, L.K.]]
+[[Category: Nicholson, L K.]]
-[[Category: Pascuzzi, P.E.]]
+[[Category: Pascuzzi, P E.]]
 [[Category: Wulf, J.]]
 [[Category: omega loop]]
 [[Category: three-helix bundle]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:10:37 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:47:15 2008''