1iy0: Difference between revisions
New page: left|200px<br /><applet load="1iy0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iy0, resolution 2.95Å" /> '''Crystal structure of... |
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[[Image:1iy0.jpg|left|200px]]<br /><applet load="1iy0" size=" | [[Image:1iy0.jpg|left|200px]]<br /><applet load="1iy0" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1iy0, resolution 2.95Å" /> | caption="1iy0, resolution 2.95Å" /> | ||
'''Crystal structure of the FtsH ATPase domain with AMP-PNP from Thermus thermophilus'''<br /> | '''Crystal structure of the FtsH ATPase domain with AMP-PNP from Thermus thermophilus'''<br /> | ||
==Overview== | ==Overview== | ||
FtsH is a cytoplasmic membrane-integrated, ATP-dependent metalloprotease, which processively degrades both cytoplasmic and membrane proteins in | FtsH is a cytoplasmic membrane-integrated, ATP-dependent metalloprotease, which processively degrades both cytoplasmic and membrane proteins in concert with unfolding. The FtsH protein is divided into the N-terminal transmembrane region and the larger C-terminal cytoplasmic region, which consists of an ATPase domain and a protease domain. We have determined the crystal structures of the Thermus thermophilus FtsH ATPase domain in the nucleotide-free and AMP-PNP- and ADP-bound states, in addition to the domain with the extra preceding segment. Combined with the mapping of the putative substrate binding region, these structures suggest that FtsH internally forms a hexameric ring structure, in which ATP binding could cause a conformational change to facilitate transport of substrates into the protease domain through the central pore. | ||
==About this Structure== | ==About this Structure== | ||
1IY0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ANP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1IY0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IY0 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: aaa domain fold]] | [[Category: aaa domain fold]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:16:50 2008'' | ||
Revision as of 11:16, 21 February 2008
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Crystal structure of the FtsH ATPase domain with AMP-PNP from Thermus thermophilus
Overview
FtsH is a cytoplasmic membrane-integrated, ATP-dependent metalloprotease, which processively degrades both cytoplasmic and membrane proteins in concert with unfolding. The FtsH protein is divided into the N-terminal transmembrane region and the larger C-terminal cytoplasmic region, which consists of an ATPase domain and a protease domain. We have determined the crystal structures of the Thermus thermophilus FtsH ATPase domain in the nucleotide-free and AMP-PNP- and ADP-bound states, in addition to the domain with the extra preceding segment. Combined with the mapping of the putative substrate binding region, these structures suggest that FtsH internally forms a hexameric ring structure, in which ATP binding could cause a conformational change to facilitate transport of substrates into the protease domain through the central pore.
About this Structure
1IY0 is a Single protein structure of sequence from Thermus thermophilus with ANP as ligand. Full crystallographic information is available from OCA.
Reference
Hexameric ring structure of the ATPase domain of the membrane-integrated metalloprotease FtsH from Thermus thermophilus HB8., Niwa H, Tsuchiya D, Makyio H, Yoshida M, Morikawa K, Structure. 2002 Oct;10(10):1415-23. PMID:12377127
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