1na3: Difference between revisions
New page: left|200px<br /><applet load="1na3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1na3, resolution 1.55Å" /> '''Design of Stable alp... |
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[[Image:1na3.jpg|left|200px]]<br /><applet load="1na3" size=" | [[Image:1na3.jpg|left|200px]]<br /><applet load="1na3" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1na3, resolution 1.55Å" /> | caption="1na3, resolution 1.55Å" /> | ||
'''Design of Stable alpha-Helical Arrays from an Idealized TPR Motif'''<br /> | '''Design of Stable alpha-Helical Arrays from an Idealized TPR Motif'''<br /> | ||
==Overview== | ==Overview== | ||
The tetratricopeptide repeat (TPR) is a 34-amino acid alpha-helical motif | The tetratricopeptide repeat (TPR) is a 34-amino acid alpha-helical motif that occurs in over 300 different proteins. In the different proteins, three to sixteen or more TPR motifs occur in tandem arrays and function to mediate protein-protein interactions. The binding specificity of each TPR protein is different, although the underlying structural motif is the same. Here we describe a statistical approach to the design of an idealized TPR motif. We present the high-resolution X-ray crystal structures (to 1.55 and 1.6 A) of designed TPR proteins and describe their solution properties and stability. A detailed analysis of these structures provides an understanding of the TPR motif, how it is repeated to give helical arrays with different superhelical twists, and how a very stable framework may be constructed for future functional designs. | ||
==About this Structure== | ==About this Structure== | ||
1NA3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG, TRS and IPT as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1NA3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=TRS:'>TRS</scene> and <scene name='pdbligand=IPT:'>IPT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NA3 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ]] | [[Category: ]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Andrea, L | [[Category: Andrea, L D.]] | ||
[[Category: Cocco, M.]] | [[Category: Cocco, M.]] | ||
[[Category: Main, E.]] | [[Category: Main, E.]] | ||
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[[Category: tpr]] | [[Category: tpr]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:54 2008'' | ||
Revision as of 12:03, 21 February 2008
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Design of Stable alpha-Helical Arrays from an Idealized TPR Motif
Overview
The tetratricopeptide repeat (TPR) is a 34-amino acid alpha-helical motif that occurs in over 300 different proteins. In the different proteins, three to sixteen or more TPR motifs occur in tandem arrays and function to mediate protein-protein interactions. The binding specificity of each TPR protein is different, although the underlying structural motif is the same. Here we describe a statistical approach to the design of an idealized TPR motif. We present the high-resolution X-ray crystal structures (to 1.55 and 1.6 A) of designed TPR proteins and describe their solution properties and stability. A detailed analysis of these structures provides an understanding of the TPR motif, how it is repeated to give helical arrays with different superhelical twists, and how a very stable framework may be constructed for future functional designs.
About this Structure
1NA3 is a Protein complex structure of sequences from [1] with MG, TRS and IPT as ligands. Full crystallographic information is available from OCA.
Reference
Design of stable alpha-helical arrays from an idealized TPR motif., Main ER, Xiong Y, Cocco MJ, D'Andrea L, Regan L, Structure. 2003 May;11(5):497-508. PMID:12737816 [[Category: ]]
Page seeded by OCA on Thu Feb 21 14:03:54 2008
