1evn: Difference between revisions

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[[Image:1evn.gif|left|200px]]<br /><applet load="1evn" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1evn.gif|left|200px]]<br /><applet load="1evn" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1evn" />

'''NMR OBSERVATION OF A-TETRAD'''<br />

==Overview==

The structure of the telomeric DNA has been a subject of extensive, investigation in recent years due to the realization that it has important, functional roles to play in vivo and the observations that truncated, telomeric sequences exhibit a great variety of 3D structures in aqueous, solutions. In this context, we describe here NMR structural studies on two, truncated human telomeric DNA sequences, d-AG(3)T and d-TAG(3)T in, solutions containing K(+)ions. The G(3)stretches in both the, oligonucleotides were seen to form parallel-stranded quadruplexes., However, the AG(3)segment as a whole, had different structural, characteristics. The structure of d-AG(3)T revealed the formation of a, novel A-tetrad, which was not seen in d-TAG(3)T. The A's in the tetrad had, syn glycosidic conformation as opposed to the anti conformation of the G's, in the G-tetrads. The A-tetrad stacked well over the adjacent G-tetrad and, the twist angle at this step was smaller in d-AG(3)T than in d-TAG(3)T., These observations are expected to be significant from the point of view, of structural diversity and recognition in telomeres.

The structure of the telomeric DNA has been a subject of extensive investigation in recent years due to the realization that it has important functional roles to play in vivo and the observations that truncated telomeric sequences exhibit a great variety of 3D structures in aqueous solutions. In this context, we describe here NMR structural studies on two truncated human telomeric DNA sequences, d-AG(3)T and d-TAG(3)T in solutions containing K(+)ions. The G(3)stretches in both the oligonucleotides were seen to form parallel-stranded quadruplexes. However, the AG(3)segment as a whole, had different structural characteristics. The structure of d-AG(3)T revealed the formation of a novel A-tetrad, which was not seen in d-TAG(3)T. The A's in the tetrad had syn glycosidic conformation as opposed to the anti conformation of the G's in the G-tetrads. The A-tetrad stacked well over the adjacent G-tetrad and the twist angle at this step was smaller in d-AG(3)T than in d-TAG(3)T. These observations are expected to be significant from the point of view of structural diversity and recognition in telomeres.

==About this Structure==

1EVN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1EVN OCA].

1EVN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EVN OCA].

==Reference==

NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad., Patel PK, Koti AS, Hosur RV, Nucleic Acids Res. 1999 Oct 1;27(19):3836-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10481022 10481022]

[[Category: Protein complex]]

[[Category: Hosur, R.V.]]

[[Category: Hosur, R V.]]

[[Category: Koti, A.S.R.]]

[[Category: Koti, A S.R.]]

[[Category: Patel, P.K.]]

[[Category: Patel, P K.]]

[[Category: a-tetrad]]

[[Category: dna]]

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[[Category: nmr structure]]

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@@ Line 1: / Line 1: @@
-[[Image:1evn.gif|left|200px]]<br /><applet load="1evn" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1evn.gif|left|200px]]<br /><applet load="1evn" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1evn" />
 '''NMR OBSERVATION OF A-TETRAD'''<br />
 ==Overview==
-The structure of the telomeric DNA has been a subject of extensive, investigation in recent years due to the realization that it has important, functional roles to play in vivo and the observations that truncated, telomeric sequences exhibit a great variety of 3D structures in aqueous, solutions. In this context, we describe here NMR structural studies on two, truncated human telomeric DNA sequences, d-AG(3)T and d-TAG(3)T in, solutions containing K(+)ions. The G(3)stretches in both the, oligonucleotides were seen to form parallel-stranded quadruplexes., However, the AG(3)segment as a whole, had different structural, characteristics. The structure of d-AG(3)T revealed the formation of a, novel A-tetrad, which was not seen in d-TAG(3)T. The A's in the tetrad had, syn glycosidic conformation as opposed to the anti conformation of the G's, in the G-tetrads. The A-tetrad stacked well over the adjacent G-tetrad and, the twist angle at this step was smaller in d-AG(3)T than in d-TAG(3)T., These observations are expected to be significant from the point of view, of structural diversity and recognition in telomeres.
+The structure of the telomeric DNA has been a subject of extensive investigation in recent years due to the realization that it has important functional roles to play in vivo and the observations that truncated telomeric sequences exhibit a great variety of 3D structures in aqueous solutions. In this context, we describe here NMR structural studies on two truncated human telomeric DNA sequences, d-AG(3)T and d-TAG(3)T in solutions containing K(+)ions. The G(3)stretches in both the oligonucleotides were seen to form parallel-stranded quadruplexes. However, the AG(3)segment as a whole, had different structural characteristics. The structure of d-AG(3)T revealed the formation of a novel A-tetrad, which was not seen in d-TAG(3)T. The A's in the tetrad had syn glycosidic conformation as opposed to the anti conformation of the G's in the G-tetrads. The A-tetrad stacked well over the adjacent G-tetrad and the twist angle at this step was smaller in d-AG(3)T than in d-TAG(3)T. These observations are expected to be significant from the point of view of structural diversity and recognition in telomeres.
 ==About this Structure==
-EVN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EVN OCA].
+EVN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EVN OCA].
 ==Reference==
 NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad., Patel PK, Koti AS, Hosur RV, Nucleic Acids Res. 1999 Oct 1;27(19):3836-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10481022 10481022]
 [[Category: Protein complex]]
-[[Category: Hosur, R.V.]]
+[[Category: Hosur, R V.]]
-[[Category: Koti, A.S.R.]]
+[[Category: Koti, A S.R.]]
-[[Category: Patel, P.K.]]
+[[Category: Patel, P K.]]
 [[Category: a-tetrad]]
 [[Category: dna]]
@@ Line 21: / Line 21: @@
 [[Category: nmr structure]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:53:34 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:31:57 2008''