1f3s: Difference between revisions

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[[Image:1f3s.gif|left|200px]]<br /><applet load="1f3s" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1f3s.gif|left|200px]]<br /><applet load="1f3s" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1f3s" />

'''SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.'''<br />

==Overview==

We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to, form a 2-fold symmetric G-quadruplex in which G(syn)., G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A), triads. The NMR-based solution structural analysis was greatly aided by, monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen, bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled, sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure, establishes that the guanine base-pairs with the cytosine through, Watson-Crick G.C pair formation and with adenine through sheared G.A, mismatch formation within the G.(C-A) triad. A model of triad DNA was, constructed that contains the experimentally determined G.(C-A) triad, alignment as the repeating stacked unit.

We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to form a 2-fold symmetric G-quadruplex in which G(syn). G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A) triads. The NMR-based solution structural analysis was greatly aided by monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure establishes that the guanine base-pairs with the cytosine through Watson-Crick G.C pair formation and with adenine through sheared G.A mismatch formation within the G.(C-A) triad. A model of triad DNA was constructed that contains the experimentally determined G.(C-A) triad alignment as the repeating stacked unit.

==About this Structure==

1F3S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA].

1F3S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA].

==Reference==

Line 16:

[[Category: Kettani, A.]]

[[Category: Majumdar, A.]]

[[Category: Patel, D.J.]]

[[Category: Patel, D J.]]

[[Category: Skripkin, E.]]

[[Category: 15n-labeled dna]]

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[[Category: uniform 13c]]

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@@ Line 1: / Line 1: @@
-[[Image:1f3s.gif|left|200px]]<br /><applet load="1f3s" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1f3s.gif|left|200px]]<br /><applet load="1f3s" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1f3s" />
 '''SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.'''<br />
 ==Overview==
-We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to, form a 2-fold symmetric G-quadruplex in which G(syn)., G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A), triads. The NMR-based solution structural analysis was greatly aided by, monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen, bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled, sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure, establishes that the guanine base-pairs with the cytosine through, Watson-Crick G.C pair formation and with adenine through sheared G.A, mismatch formation within the G.(C-A) triad. A model of triad DNA was, constructed that contains the experimentally determined G.(C-A) triad, alignment as the repeating stacked unit.
+We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to form a 2-fold symmetric G-quadruplex in which G(syn). G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A) triads. The NMR-based solution structural analysis was greatly aided by monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure establishes that the guanine base-pairs with the cytosine through Watson-Crick G.C pair formation and with adenine through sheared G.A mismatch formation within the G.(C-A) triad. A model of triad DNA was constructed that contains the experimentally determined G.(C-A) triad alignment as the repeating stacked unit.
 ==About this Structure==
-F3S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA].
+F3S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Kettani, A.]]
 [[Category: Majumdar, A.]]
-[[Category: Patel, D.J.]]
+[[Category: Patel, D J.]]
 [[Category: Skripkin, E.]]
 [[Category: 15n-labeled dna]]
@@ Line 24: / Line 24: @@
 [[Category: uniform 13c]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:14:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:34:29 2008''