1f85: Difference between revisions

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[[Image:1f85.gif|left|200px]]<br /><applet load="1f85" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1f85.gif|left|200px]]<br /><applet load="1f85" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1f85" />

'''SOLUTION STRUCTURE OF HCV IRES RNA DOMAIN IIIE'''<br />

==Overview==

Translation of the hepatitis C virus (HCV) polyprotein is initiated at an, internal ribosome entry site (IRES) element in the 5' untranslated region, of HCV RNA. The HCV IRES element interacts directly with the 40S subunit, and biochemical experiments have implicated RNA elements near the AUG, start codon as required for IRES-40S subunit complex formation. The data, we present here show that two RNA stem loops, domains IIId and IIIe, are, involved in IRES-40S subunit interaction. The structures of the two RNA, domains were solved by NMR spectroscopy and reveal structural features, that may explain their role in IRES function.

Translation of the hepatitis C virus (HCV) polyprotein is initiated at an internal ribosome entry site (IRES) element in the 5' untranslated region of HCV RNA. The HCV IRES element interacts directly with the 40S subunit, and biochemical experiments have implicated RNA elements near the AUG start codon as required for IRES-40S subunit complex formation. The data we present here show that two RNA stem loops, domains IIId and IIIe, are involved in IRES-40S subunit interaction. The structures of the two RNA domains were solved by NMR spectroscopy and reveal structural features that may explain their role in IRES function.

==About this Structure==

1F85 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1F85 OCA].

1F85 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F85 OCA].

==Reference==

Structures of two RNA domains essential for hepatitis C virus internal ribosome entry site function., Lukavsky PJ, Otto GA, Lancaster AM, Sarnow P, Puglisi JD, Nat Struct Biol. 2000 Dec;7(12):1105-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11101890 11101890]

[[Category: Protein complex]]

[[Category: Lancaster, A.M.]]

[[Category: Lancaster, A M.]]

[[Category: Lukavsky, P.J.]]

[[Category: Lukavsky, P J.]]

[[Category: Otto, G.A.]]

[[Category: Otto, G A.]]

[[Category: Puglisi, J.D.]]

[[Category: Puglisi, J D.]]

[[Category: Sarnow, P.]]

[[Category: hairpin loop]]

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[[Category: tetraloop]]

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@@ Line 1: / Line 1: @@
-[[Image:1f85.gif|left|200px]]<br /><applet load="1f85" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1f85.gif|left|200px]]<br /><applet load="1f85" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1f85" />
 '''SOLUTION STRUCTURE OF HCV IRES RNA DOMAIN IIIE'''<br />
 ==Overview==
-Translation of the hepatitis C virus (HCV) polyprotein is initiated at an, internal ribosome entry site (IRES) element in the 5' untranslated region, of HCV RNA. The HCV IRES element interacts directly with the 40S subunit, and biochemical experiments have implicated RNA elements near the AUG, start codon as required for IRES-40S subunit complex formation. The data, we present here show that two RNA stem loops, domains IIId and IIIe, are, involved in IRES-40S subunit interaction. The structures of the two RNA, domains were solved by NMR spectroscopy and reveal structural features, that may explain their role in IRES function.
+Translation of the hepatitis C virus (HCV) polyprotein is initiated at an internal ribosome entry site (IRES) element in the 5' untranslated region of HCV RNA. The HCV IRES element interacts directly with the 40S subunit, and biochemical experiments have implicated RNA elements near the AUG start codon as required for IRES-40S subunit complex formation. The data we present here show that two RNA stem loops, domains IIId and IIIe, are involved in IRES-40S subunit interaction. The structures of the two RNA domains were solved by NMR spectroscopy and reveal structural features that may explain their role in IRES function.
 ==About this Structure==
-F85 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F85 OCA].
+F85 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F85 OCA].
 ==Reference==
 Structures of two RNA domains essential for hepatitis C virus internal ribosome entry site function., Lukavsky PJ, Otto GA, Lancaster AM, Sarnow P, Puglisi JD, Nat Struct Biol. 2000 Dec;7(12):1105-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11101890 11101890]
 [[Category: Protein complex]]
-[[Category: Lancaster, A.M.]]
+[[Category: Lancaster, A M.]]
-[[Category: Lukavsky, P.J.]]
+[[Category: Lukavsky, P J.]]
-[[Category: Otto, G.A.]]
+[[Category: Otto, G A.]]
-[[Category: Puglisi, J.D.]]
+[[Category: Puglisi, J D.]]
 [[Category: Sarnow, P.]]
 [[Category: hairpin loop]]
@@ Line 23: / Line 23: @@
 [[Category: tetraloop]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:28:21 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:35:54 2008''