1rz9: Difference between revisions

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[[Image:1rz9.gif|left|200px]]<br /><applet load="1rz9" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1rz9.gif|left|200px]]<br /><applet load="1rz9" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1rz9, resolution 3.10Å" />

'''Crystal Structure of AAV Rep complexed with the Rep-binding sequence'''<br />

==Overview==

Integration into a particular location in human chromosomes is a unique, property of the adeno-associated virus (AAV). This reaction requires the, viral Rep protein and AAV origin sequences. To understand how Rep, recognizes DNA, we have determined the structures of the Rep endonuclease, domain separately complexed with two DNA substrates: the Rep binding site, within the viral inverted terminal repeat and one of the terminal hairpin, arms. At the Rep binding site, five Rep monomers bind five tetranucleotide, direct repeats; each repeat is recognized by two Rep monomers from, opposing faces of the DNA. Stem-loop binding involves a protein interface, on the opposite side of the molecule from the active site where ssDNA is, cleaved. Rep therefore has three distinct binding sites within its, endonuclease domain for its different DNA substrates. Use of these, different interfaces generates the structural asymmetry necessary to, regulate later events in viral replication and integration.

Integration into a particular location in human chromosomes is a unique property of the adeno-associated virus (AAV). This reaction requires the viral Rep protein and AAV origin sequences. To understand how Rep recognizes DNA, we have determined the structures of the Rep endonuclease domain separately complexed with two DNA substrates: the Rep binding site within the viral inverted terminal repeat and one of the terminal hairpin arms. At the Rep binding site, five Rep monomers bind five tetranucleotide direct repeats; each repeat is recognized by two Rep monomers from opposing faces of the DNA. Stem-loop binding involves a protein interface on the opposite side of the molecule from the active site where ssDNA is cleaved. Rep therefore has three distinct binding sites within its endonuclease domain for its different DNA substrates. Use of these different interfaces generates the structural asymmetry necessary to regulate later events in viral replication and integration.

==About this Structure==

1RZ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA].

1RZ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Dyda, F.]]

[[Category: Hickman, A.B.]]

[[Category: Hickman, A B.]]

[[Category: Kotin, R.M.]]

[[Category: Kotin, R M.]]

[[Category: Perez, Z.N.]]

[[Category: Perez, Z N.]]

[[Category: Ronning, D.R.]]

[[Category: Ronning, D R.]]

[[Category: protein-dna complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sat Nov 24 23~~:35:~~02 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:18 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1rz9.gif|left|200px]]<br /><applet load="1rz9" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1rz9.gif|left|200px]]<br /><applet load="1rz9" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1rz9, resolution 3.10&Aring;" />
 '''Crystal Structure of AAV Rep complexed with the Rep-binding sequence'''<br />
 ==Overview==
-Integration into a particular location in human chromosomes is a unique, property of the adeno-associated virus (AAV). This reaction requires the, viral Rep protein and AAV origin sequences. To understand how Rep, recognizes DNA, we have determined the structures of the Rep endonuclease, domain separately complexed with two DNA substrates: the Rep binding site, within the viral inverted terminal repeat and one of the terminal hairpin, arms. At the Rep binding site, five Rep monomers bind five tetranucleotide, direct repeats; each repeat is recognized by two Rep monomers from, opposing faces of the DNA. Stem-loop binding involves a protein interface, on the opposite side of the molecule from the active site where ssDNA is, cleaved. Rep therefore has three distinct binding sites within its, endonuclease domain for its different DNA substrates. Use of these, different interfaces generates the structural asymmetry necessary to, regulate later events in viral replication and integration.
+Integration into a particular location in human chromosomes is a unique property of the adeno-associated virus (AAV). This reaction requires the viral Rep protein and AAV origin sequences. To understand how Rep recognizes DNA, we have determined the structures of the Rep endonuclease domain separately complexed with two DNA substrates: the Rep binding site within the viral inverted terminal repeat and one of the terminal hairpin arms. At the Rep binding site, five Rep monomers bind five tetranucleotide direct repeats; each repeat is recognized by two Rep monomers from opposing faces of the DNA. Stem-loop binding involves a protein interface on the opposite side of the molecule from the active site where ssDNA is cleaved. Rep therefore has three distinct binding sites within its endonuclease domain for its different DNA substrates. Use of these different interfaces generates the structural asymmetry necessary to regulate later events in viral replication and integration.
 ==About this Structure==
-RZ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA].
+RZ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Dyda, F.]]
-[[Category: Hickman, A.B.]]
+[[Category: Hickman, A B.]]
-[[Category: Kotin, R.M.]]
+[[Category: Kotin, R M.]]
-[[Category: Perez, Z.N.]]
+[[Category: Perez, Z N.]]
-[[Category: Ronning, D.R.]]
+[[Category: Ronning, D R.]]
 [[Category: protein-dna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:35:02 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:18 2008''