1jml: Difference between revisions

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[[Image:1jml.jpg|left|200px]]<br /><applet load="1jml" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1jml.jpg|left|200px]]<br /><applet load="1jml" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1jml, resolution 1.90Å" />

'''Conversion of Monomeric Protein L to an Obligate Dimer by Computational Protein Design'''<br />

==Overview==

Protein L consists of a single alpha-helix packed on a four-stranded, beta-sheet formed by two symmetrically opposed beta-hairpins. We use a, computer-based protein design procedure to stabilize a domain-swapped, dimer of protein L in which the second beta-turn straightens and the, C-terminal strand inserts into the beta-sheet of the partner. The designed, obligate dimer contains three mutations (A52V, N53P, and G55A) and has a, dissociation constant of approximately 700 pM, which is comparable to the, dissociation constant of many naturally occurring protein dimers. The, structure of the dimer has been determined by x-ray crystallography and is, close to the in silico model.

Protein L consists of a single alpha-helix packed on a four-stranded beta-sheet formed by two symmetrically opposed beta-hairpins. We use a computer-based protein design procedure to stabilize a domain-swapped dimer of protein L in which the second beta-turn straightens and the C-terminal strand inserts into the beta-sheet of the partner. The designed obligate dimer contains three mutations (A52V, N53P, and G55A) and has a dissociation constant of approximately 700 pM, which is comparable to the dissociation constant of many naturally occurring protein dimers. The structure of the dimer has been determined by x-ray crystallography and is close to the in silico model.

==About this Structure==

1JML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1JML OCA].

1JML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JML OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Baker, D.]]

[[Category: Kim, D.E.]]

[[Category: Kim, D E.]]

[[Category: Kuhlman, B]]

[[Category: Neill, J.W.O.]]

[[Category: Neill, J W.O.]]

[[Category: Zhang, K.Y.J.]]

[[Category: Zhang, K Y.J.]]

[[Category: ZN]]

[[Category: carboxy-terminal beta-strand swapped.]]

Line 23:

[[Category: four stranded beta-sheet with central alpha helix]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sat Nov~~ 24 ~~23:52~~:~~50 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:21 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1jml.jpg|left|200px]]<br /><applet load="1jml" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jml.jpg|left|200px]]<br /><applet load="1jml" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1jml, resolution 1.90&Aring;" />
 '''Conversion of Monomeric Protein L to an Obligate Dimer by Computational Protein Design'''<br />
 ==Overview==
-Protein L consists of a single alpha-helix packed on a four-stranded, beta-sheet formed by two symmetrically opposed beta-hairpins. We use a, computer-based protein design procedure to stabilize a domain-swapped, dimer of protein L in which the second beta-turn straightens and the, C-terminal strand inserts into the beta-sheet of the partner. The designed, obligate dimer contains three mutations (A52V, N53P, and G55A) and has a, dissociation constant of approximately 700 pM, which is comparable to the, dissociation constant of many naturally occurring protein dimers. The, structure of the dimer has been determined by x-ray crystallography and is, close to the in silico model.
+Protein L consists of a single alpha-helix packed on a four-stranded beta-sheet formed by two symmetrically opposed beta-hairpins. We use a computer-based protein design procedure to stabilize a domain-swapped dimer of protein L in which the second beta-turn straightens and the C-terminal strand inserts into the beta-sheet of the partner. The designed obligate dimer contains three mutations (A52V, N53P, and G55A) and has a dissociation constant of approximately 700 pM, which is comparable to the dissociation constant of many naturally occurring protein dimers. The structure of the dimer has been determined by x-ray crystallography and is close to the in silico model.
 ==About this Structure==
-JML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JML OCA].
+JML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JML OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Baker, D.]]
-[[Category: Kim, D.E.]]
+[[Category: Kim, D E.]]
 [[Category: Kuhlman, B]]
-[[Category: Neill, J.W.O.]]
+[[Category: Neill, J W.O.]]
-[[Category: Zhang, K.Y.J.]]
+[[Category: Zhang, K Y.J.]]
 [[Category: ZN]]
 [[Category: carboxy-terminal beta-strand swapped.]]
@@ Line 23: / Line 23: @@
 [[Category: four stranded beta-sheet with central alpha helix]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:52:50 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:21 2008''