1fml: Difference between revisions

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[[Image:1fml.gif|left|200px]]<br /><applet load="1fml" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1fml.gif|left|200px]]<br /><applet load="1fml" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1fml, resolution 2.75Å" />

'''CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP'''<br />

==Overview==

We report here the crystal structure of retinol dehydratase, an enzyme, that catalyzes the synthesis of anhydroretinol. The enzyme is a member of, the sulfotransferase superfamily and its crystal structure reveals the, insertion of a helical lid into a canonical sulfotransferase fold., Site-directed mutations demonstrate that this inserted lid is necessary, for anhydroretinol production but not for sulfonation; thus, insertion of, a helical lid can convert a sulfotransferase into a dehydratase.

We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.

==About this Structure==

1FML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda] with CA, A3P and RTL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1FML OCA].

1FML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=A3P:'>A3P</scene> and <scene name='pdbligand=RTL:'>RTL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FML OCA].

==Reference==

Line 15:

[[Category: Buck, J.]]

[[Category: Kobayashi, M.]]

[[Category: Newcomer, M.E.]]

[[Category: Newcomer, M E.]]

[[Category: Pakhomova, S.]]

[[Category: A3P]]

Line 26:

[[Category: sulfotransferase]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:40:16 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1fml.gif|left|200px]]<br /><applet load="1fml" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1fml.gif|left|200px]]<br /><applet load="1fml" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1fml, resolution 2.75&Aring;" />
 '''CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP'''<br />
 ==Overview==
-We report here the crystal structure of retinol dehydratase, an enzyme, that catalyzes the synthesis of anhydroretinol. The enzyme is a member of, the sulfotransferase superfamily and its crystal structure reveals the, insertion of a helical lid into a canonical sulfotransferase fold., Site-directed mutations demonstrate that this inserted lid is necessary, for anhydroretinol production but not for sulfonation; thus, insertion of, a helical lid can convert a sulfotransferase into a dehydratase.
+We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.
 ==About this Structure==
-FML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda] with CA, A3P and RTL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FML OCA].
+FML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=A3P:'>A3P</scene> and <scene name='pdbligand=RTL:'>RTL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FML OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Buck, J.]]
 [[Category: Kobayashi, M.]]
-[[Category: Newcomer, M.E.]]
+[[Category: Newcomer, M E.]]
 [[Category: Pakhomova, S.]]
 [[Category: A3P]]
@@ Line 26: / Line 26: @@
 [[Category: sulfotransferase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:03:28 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:40:16 2008''