1s9h: Difference between revisions
New page: left|200px<br /><applet load="1s9h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s9h, resolution 2.40Å" /> '''Crystal Structure of... |
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[[Image:1s9h.gif|left|200px]]<br /><applet load="1s9h" size=" | [[Image:1s9h.gif|left|200px]]<br /><applet load="1s9h" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1s9h, resolution 2.40Å" /> | caption="1s9h, resolution 2.40Å" /> | ||
'''Crystal Structure of Adeno-associated virus Type 2 Rep40'''<br /> | '''Crystal Structure of Adeno-associated virus Type 2 Rep40'''<br /> | ||
==Overview== | ==Overview== | ||
We report here the crystal structure of an SF3 DNA helicase, Rep40, from | We report here the crystal structure of an SF3 DNA helicase, Rep40, from adeno-associated virus 2 (AAV2). We show that AAV2 Rep40 is structurally more similar to the AAA(+) class of cellular proteins than to DNA helicases from other superfamilies. The structure delineates the expected Walker A and B motifs, but also reveals an unexpected "arginine finger" that directly implies the requirement of Rep40 oligomerization for ATP hydrolysis and helicase activity. Further, the Rep40 AAA(+) domain is novel in that it is unimodular as opposed to bimodular. Altogether, the structural connection to AAA(+) proteins defines the general architecture of SF3 DNA helicases, a family that includes simian virus 40 (SV40) T antigen, as well as provides a conceptual framework for understanding the role of Rep proteins during AAV DNA replication, packaging, and site-specific integration. | ||
==About this Structure== | ==About this Structure== | ||
1S9H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_2 Adeno-associated virus 2]. Full crystallographic information is available from [http:// | 1S9H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_2 Adeno-associated virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9H OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Adeno-associated virus 2]] | [[Category: Adeno-associated virus 2]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Aggarwal, A | [[Category: Aggarwal, A K.]] | ||
[[Category: Edwards, T | [[Category: Edwards, T A.]] | ||
[[Category: Escalante, C | [[Category: Escalante, C R.]] | ||
[[Category: James, J | [[Category: James, J A.]] | ||
[[Category: Linden, R | [[Category: Linden, R M.]] | ||
[[Category: Yoon-Robarts, M.]] | [[Category: Yoon-Robarts, M.]] | ||
[[Category: aaa+ protein]] | [[Category: aaa+ protein]] | ||
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[[Category: walker b]] | [[Category: walker b]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:19 2008'' | ||
Revision as of 12:59, 21 February 2008
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Crystal Structure of Adeno-associated virus Type 2 Rep40
Overview
We report here the crystal structure of an SF3 DNA helicase, Rep40, from adeno-associated virus 2 (AAV2). We show that AAV2 Rep40 is structurally more similar to the AAA(+) class of cellular proteins than to DNA helicases from other superfamilies. The structure delineates the expected Walker A and B motifs, but also reveals an unexpected "arginine finger" that directly implies the requirement of Rep40 oligomerization for ATP hydrolysis and helicase activity. Further, the Rep40 AAA(+) domain is novel in that it is unimodular as opposed to bimodular. Altogether, the structural connection to AAA(+) proteins defines the general architecture of SF3 DNA helicases, a family that includes simian virus 40 (SV40) T antigen, as well as provides a conceptual framework for understanding the role of Rep proteins during AAV DNA replication, packaging, and site-specific integration.
About this Structure
1S9H is a Single protein structure of sequence from Adeno-associated virus 2. Full crystallographic information is available from OCA.
Reference
Crystal structure of the SF3 helicase from adeno-associated virus type 2., James JA, Escalante CR, Yoon-Robarts M, Edwards TA, Linden RM, Aggarwal AK, Structure. 2003 Aug;11(8):1025-35. PMID:12906833
Page seeded by OCA on Thu Feb 21 14:59:19 2008
