1s9l: Difference between revisions

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[[Image:1s9l.jpg|left|200px]]<br /><applet load="1s9l" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1s9l.jpg|left|200px]]<br /><applet load="1s9l" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1s9l" />

'''NMR Solution Structure of a Parallel LNA Quadruplex'''<br />

==Overview==

The solution structure of a locked nucleic acid (LNA) quadruplex, formed, by the oligomer d(TGGGT), containing only conformationally restricted LNA, residues is reported. NMR and CD spectroscopy, as well as molecular, dynamics and mechanic calculations, has been used to characterize the, complex. The molecule adopts a parallel stranded conformation with a, 4-fold rotational symmetry, showing a right-handed helicity and the, guanine residues in an almost planar conformation with three well-defined, G-tetrads. The thermal stability of Q-LNA has been found to be comparable, with that of [r(UGGGU)]4, while a T(m) increment of 20 degrees C with, respect to the corresponding DNA quadruplex structure [d(TGGGT)]4 has been, observed. The structural features of the LNA quadruplex reported here may, open new perspectives for the biological application of LNAs as novel, versatile tools to design aptamer or catalyst oligonucleotides.

The solution structure of a locked nucleic acid (LNA) quadruplex, formed by the oligomer d(TGGGT), containing only conformationally restricted LNA residues is reported. NMR and CD spectroscopy, as well as molecular dynamics and mechanic calculations, has been used to characterize the complex. The molecule adopts a parallel stranded conformation with a 4-fold rotational symmetry, showing a right-handed helicity and the guanine residues in an almost planar conformation with three well-defined G-tetrads. The thermal stability of Q-LNA has been found to be comparable with that of [r(UGGGU)]4, while a T(m) increment of 20 degrees C with respect to the corresponding DNA quadruplex structure [d(TGGGT)]4 has been observed. The structural features of the LNA quadruplex reported here may open new perspectives for the biological application of LNAs as novel versatile tools to design aptamer or catalyst oligonucleotides.

==About this Structure==

1S9L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1S9L OCA].

1S9L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9L OCA].

==Reference==

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[[Category: quadruplex]]

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@@ Line 1: / Line 1: @@
-[[Image:1s9l.jpg|left|200px]]<br /><applet load="1s9l" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1s9l.jpg|left|200px]]<br /><applet load="1s9l" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1s9l" />
 '''NMR Solution Structure of a Parallel LNA Quadruplex'''<br />
 ==Overview==
-The solution structure of a locked nucleic acid (LNA) quadruplex, formed, by the oligomer d(TGGGT), containing only conformationally restricted LNA, residues is reported. NMR and CD spectroscopy, as well as molecular, dynamics and mechanic calculations, has been used to characterize the, complex. The molecule adopts a parallel stranded conformation with a, 4-fold rotational symmetry, showing a right-handed helicity and the, guanine residues in an almost planar conformation with three well-defined, G-tetrads. The thermal stability of Q-LNA has been found to be comparable, with that of [r(UGGGU)]4, while a T(m) increment of 20 degrees C with, respect to the corresponding DNA quadruplex structure [d(TGGGT)]4 has been, observed. The structural features of the LNA quadruplex reported here may, open new perspectives for the biological application of LNAs as novel, versatile tools to design aptamer or catalyst oligonucleotides.
+The solution structure of a locked nucleic acid (LNA) quadruplex, formed by the oligomer d(TGGGT), containing only conformationally restricted LNA residues is reported. NMR and CD spectroscopy, as well as molecular dynamics and mechanic calculations, has been used to characterize the complex. The molecule adopts a parallel stranded conformation with a 4-fold rotational symmetry, showing a right-handed helicity and the guanine residues in an almost planar conformation with three well-defined G-tetrads. The thermal stability of Q-LNA has been found to be comparable with that of [r(UGGGU)]4, while a T(m) increment of 20 degrees C with respect to the corresponding DNA quadruplex structure [d(TGGGT)]4 has been observed. The structural features of the LNA quadruplex reported here may open new perspectives for the biological application of LNAs as novel versatile tools to design aptamer or catalyst oligonucleotides.
 ==About this Structure==
-S9L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S9L OCA].
+S9L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9L OCA].
 ==Reference==
@@ Line 20: / Line 20: @@
 [[Category: quadruplex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:07:27 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:16 2008''