1s9s: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="1s9s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s9s" /> '''SOLUTION STRUCTURE OF MLV PSI SITE'''<br /> ... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1s9s.gif|left|200px]]<br /><applet load="1s9s" size=" | [[Image:1s9s.gif|left|200px]]<br /><applet load="1s9s" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1s9s" /> | caption="1s9s" /> | ||
'''SOLUTION STRUCTURE OF MLV PSI SITE'''<br /> | '''SOLUTION STRUCTURE OF MLV PSI SITE'''<br /> | ||
==Overview== | ==Overview== | ||
The full length, positive-strand genome of the Moloney Murine Leukemia | The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly. We have determined the structure of a 101-nucleotide RNA that contains this signal (called mPsi) using a novel isotope-edited NMR approach. The method is robust and should be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide linker. The structure contains five mismatched base-pairs, an unusual C.CG base-triple platform, and a novel "A-minor K-turn," in which unpaired adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond with the phosphodiester of A341. Phylogenetic analyses indicate that these essential structural elements are conserved among the murine C-type retroviruses. | ||
==About this Structure== | ==About this Structure== | ||
1S9S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1S9S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9S OCA]. | ||
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Dey, A.]] | [[Category: Dey, A.]] | ||
[[Category: Habib, D.]] | [[Category: Habib, D.]] | ||
[[Category: Souza, V | [[Category: Souza, V D.]] | ||
[[Category: Summers, M | [[Category: Summers, M F.]] | ||
[[Category: a-c mismatch]] | [[Category: a-c mismatch]] | ||
[[Category: a-minor k-turn]] | [[Category: a-minor k-turn]] | ||
| Line 25: | Line 25: | ||
[[Category: u-u mismatch]] | [[Category: u-u mismatch]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:23 2008'' | ||
