1sa9: Difference between revisions

Line 1:

[[Image:1sa9.gif|left|200px]]<br /><applet load="1sa9" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1sa9.gif|left|200px]]<br /><applet load="1sa9" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1sa9, resolution 2.86Å" />

'''Crystal Structure of the RNA octamer GGCGAGCC'''<br />

==Overview==

The crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and, 5'-GIC(GA)GCC-3', have been determined from X-ray diffraction data to 2.8, and 2.7 A resolution, respectively. The RNA octamers crystallize in, isomorphous unit cells containing two mispairs arranged in a, self-complementary manner and one single strand in the asymmetric unit., The single strand pairs with another single strand related by, crystallographic symmetry to form a third unique double helix. Tandem, non-Watson-Crick G.A/A.G base pairs of the sheared type comprise an, internal loop in the middle of each duplex. The NMR structure of this, octameric RNA sequence is also known, allowing comparison of the variation, between the six crystallographic duplexes and the solution structure. In, the symmetric duplex of the octamer containing inosine, the sheared G.A, pairs incorporate a bound water molecule. This duplex also binds one water, molecule per strand in the minor groove adjacent to the G.A pairs.

The crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and 5'-GIC(GA)GCC-3', have been determined from X-ray diffraction data to 2.8 and 2.7 A resolution, respectively. The RNA octamers crystallize in isomorphous unit cells containing two mispairs arranged in a self-complementary manner and one single strand in the asymmetric unit. The single strand pairs with another single strand related by crystallographic symmetry to form a third unique double helix. Tandem non-Watson-Crick G.A/A.G base pairs of the sheared type comprise an internal loop in the middle of each duplex. The NMR structure of this octameric RNA sequence is also known, allowing comparison of the variation between the six crystallographic duplexes and the solution structure. In the symmetric duplex of the octamer containing inosine, the sheared G.A pairs incorporate a bound water molecule. This duplex also binds one water molecule per strand in the minor groove adjacent to the G.A pairs.

==About this Structure==

1SA9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1SA9 OCA].

1SA9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SA9 OCA].

==Reference==

Line 13:

[[Category: Protein complex]]

[[Category: Baeyens, K.]]

[[Category: Holbrook, S.R.]]

[[Category: Holbrook, S R.]]

[[Category: Jang, S.B.]]

[[Category: Jang, S B.]]

[[Category: Jeong, M.S.]]

[[Category: Jeong, M S.]]

[[Category: Jr, J.SantaLucia.]]

[[Category: Jr, J SantaLucia.]]

[[Category: Turner, D.]]

[[Category: rna double helix]]

[[Category: rna tandem g-a base pair]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 00~~:09:~~50 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:28 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1sa9.gif|left|200px]]<br /><applet load="1sa9" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1sa9.gif|left|200px]]<br /><applet load="1sa9" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1sa9, resolution 2.86&Aring;" />
 '''Crystal Structure of the RNA octamer GGCGAGCC'''<br />
 ==Overview==
-The crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and, 5'-GIC(GA)GCC-3', have been determined from X-ray diffraction data to 2.8, and 2.7 A resolution, respectively. The RNA octamers crystallize in, isomorphous unit cells containing two mispairs arranged in a, self-complementary manner and one single strand in the asymmetric unit., The single strand pairs with another single strand related by, crystallographic symmetry to form a third unique double helix. Tandem, non-Watson-Crick G.A/A.G base pairs of the sheared type comprise an, internal loop in the middle of each duplex. The NMR structure of this, octameric RNA sequence is also known, allowing comparison of the variation, between the six crystallographic duplexes and the solution structure. In, the symmetric duplex of the octamer containing inosine, the sheared G.A, pairs incorporate a bound water molecule. This duplex also binds one water, molecule per strand in the minor groove adjacent to the G.A pairs.
+The crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and 5'-GIC(GA)GCC-3', have been determined from X-ray diffraction data to 2.8 and 2.7 A resolution, respectively. The RNA octamers crystallize in isomorphous unit cells containing two mispairs arranged in a self-complementary manner and one single strand in the asymmetric unit. The single strand pairs with another single strand related by crystallographic symmetry to form a third unique double helix. Tandem non-Watson-Crick G.A/A.G base pairs of the sheared type comprise an internal loop in the middle of each duplex. The NMR structure of this octameric RNA sequence is also known, allowing comparison of the variation between the six crystallographic duplexes and the solution structure. In the symmetric duplex of the octamer containing inosine, the sheared G.A pairs incorporate a bound water molecule. This duplex also binds one water molecule per strand in the minor groove adjacent to the G.A pairs.
 ==About this Structure==
-SA9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SA9 OCA].
+SA9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SA9 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Baeyens, K.]]
-[[Category: Holbrook, S.R.]]
+[[Category: Holbrook, S R.]]
-[[Category: Jang, S.B.]]
+[[Category: Jang, S B.]]
-[[Category: Jeong, M.S.]]
+[[Category: Jeong, M S.]]
-[[Category: Jr, J.SantaLucia.]]
+[[Category: Jr, J SantaLucia.]]
 [[Category: Turner, D.]]
 [[Category: rna double helix]]
 [[Category: rna tandem g-a base pair]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:09:50 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:28 2008''