1k2e: Difference between revisions

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[[Image:1k2e.jpg|left|200px]]<br /><applet load="1k2e" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1k2e.jpg|left|200px]]<br /><applet load="1k2e" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1k2e, resolution 1.80Å" />

'''crystal structure of a nudix protein from Pyrobaculum aerophilum'''<br />

==Overview==

Nudix proteins, formerly called MutT homolog proteins, are a large family, of proteins that play an important role in reducing the accumulation of, potentially toxic compounds inside the cell. They hydrolyze a wide variety, of substrates that are mainly composed of a nucleoside diphosphate linked, to some other moiety X and thus are called Nudix hydrolases. Here, the, crystal structure of a Nudix hydrolase from the hyperthermophilic archaeon, Pyrobaculum aerophilum is reported. The structure was determined by the, single-wavelength anomalous scattering method with data collected at the, peak anomalous wavelength of an iridium-derivatized crystal. It reveals an, extensive dimer interface, with each subunit contributing two strands to, the beta-sheet of the other subunit. Individual subunits consist of a, mixed highly twisted and curved beta-sheet of 11 beta-strands and two, alpha-helices, forming an alpha-beta-alpha sandwich. The conserved Nudix, box signature motif, which contains the essential catalytic residues, is, located at the first alpha-helix and the beta-strand and loop preceding, it. The unusually short connections between secondary-structural elements, together with the dimer form of the structure, are likely to contribute to, the thermostability of the P. aerophilum Nudix protein.

Nudix proteins, formerly called MutT homolog proteins, are a large family of proteins that play an important role in reducing the accumulation of potentially toxic compounds inside the cell. They hydrolyze a wide variety of substrates that are mainly composed of a nucleoside diphosphate linked to some other moiety X and thus are called Nudix hydrolases. Here, the crystal structure of a Nudix hydrolase from the hyperthermophilic archaeon Pyrobaculum aerophilum is reported. The structure was determined by the single-wavelength anomalous scattering method with data collected at the peak anomalous wavelength of an iridium-derivatized crystal. It reveals an extensive dimer interface, with each subunit contributing two strands to the beta-sheet of the other subunit. Individual subunits consist of a mixed highly twisted and curved beta-sheet of 11 beta-strands and two alpha-helices, forming an alpha-beta-alpha sandwich. The conserved Nudix box signature motif, which contains the essential catalytic residues, is located at the first alpha-helix and the beta-strand and loop preceding it. The unusually short connections between secondary-structural elements, together with the dimer form of the structure, are likely to contribute to the thermostability of the P. aerophilum Nudix protein.

==About this Structure==

1K2E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum] with SO4, NI, GOL and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1K2E OCA].

1K2E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=GOL:'>GOL</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2E OCA].

==Reference==

Line 16:

[[Category: Eisenberg, D.]]

[[Category: Mura, C.]]

[[Category: Sawaya, M.R.]]

[[Category: Sawaya, M R.]]

[[Category: Wang, S.]]

[[Category: ACY]]

Line 28:

[[Category: structural genomics]]

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@@ Line 1: / Line 1: @@
-[[Image:1k2e.jpg|left|200px]]<br /><applet load="1k2e" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1k2e.jpg|left|200px]]<br /><applet load="1k2e" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1k2e, resolution 1.80&Aring;" />
 '''crystal structure of a nudix protein from Pyrobaculum aerophilum'''<br />
 ==Overview==
-Nudix proteins, formerly called MutT homolog proteins, are a large family, of proteins that play an important role in reducing the accumulation of, potentially toxic compounds inside the cell. They hydrolyze a wide variety, of substrates that are mainly composed of a nucleoside diphosphate linked, to some other moiety X and thus are called Nudix hydrolases. Here, the, crystal structure of a Nudix hydrolase from the hyperthermophilic archaeon, Pyrobaculum aerophilum is reported. The structure was determined by the, single-wavelength anomalous scattering method with data collected at the, peak anomalous wavelength of an iridium-derivatized crystal. It reveals an, extensive dimer interface, with each subunit contributing two strands to, the beta-sheet of the other subunit. Individual subunits consist of a, mixed highly twisted and curved beta-sheet of 11 beta-strands and two, alpha-helices, forming an alpha-beta-alpha sandwich. The conserved Nudix, box signature motif, which contains the essential catalytic residues, is, located at the first alpha-helix and the beta-strand and loop preceding, it. The unusually short connections between secondary-structural elements, together with the dimer form of the structure, are likely to contribute to, the thermostability of the P. aerophilum Nudix protein.
+Nudix proteins, formerly called MutT homolog proteins, are a large family of proteins that play an important role in reducing the accumulation of potentially toxic compounds inside the cell. They hydrolyze a wide variety of substrates that are mainly composed of a nucleoside diphosphate linked to some other moiety X and thus are called Nudix hydrolases. Here, the crystal structure of a Nudix hydrolase from the hyperthermophilic archaeon Pyrobaculum aerophilum is reported. The structure was determined by the single-wavelength anomalous scattering method with data collected at the peak anomalous wavelength of an iridium-derivatized crystal. It reveals an extensive dimer interface, with each subunit contributing two strands to the beta-sheet of the other subunit. Individual subunits consist of a mixed highly twisted and curved beta-sheet of 11 beta-strands and two alpha-helices, forming an alpha-beta-alpha sandwich. The conserved Nudix box signature motif, which contains the essential catalytic residues, is located at the first alpha-helix and the beta-strand and loop preceding it. The unusually short connections between secondary-structural elements, together with the dimer form of the structure, are likely to contribute to the thermostability of the P. aerophilum Nudix protein.
 ==About this Structure==
-K2E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum] with SO4, NI, GOL and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K2E OCA].
+K2E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=GOL:'>GOL</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2E OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Eisenberg, D.]]
 [[Category: Mura, C.]]
-[[Category: Sawaya, M.R.]]
+[[Category: Sawaya, M R.]]
 [[Category: Wang, S.]]
 [[Category: ACY]]
@@ Line 28: / Line 28: @@
 [[Category: structural genomics]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:36:40 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:20 2008''